CD55 Human

CD55 is a 70-75 kDa protein that is ubiquitously expressed on the surface of cells exposed to plasma complement proteins . It functions by binding to complement proteins and accelerating their decay, thereby disrupting the complement cascade and preventing damage to host cells . This protective mechanism is essential for preventing the immune system from attacking the body’s own cells.

Recombinant human CD55 is produced using various expression systems, such as HEK293 cells or mouse myeloma cell lines . The recombinant protein is typically purified to a high degree of purity (>95%) and is often tagged with a 6×His tag for ease of purification . The recombinant form retains the functional properties of the native protein, including its ability to bind complement proteins and accelerate their decay.

Recombinant CD55 is used in various research applications, including studies on the complement system, immune response, and cell protection mechanisms. It is also used in functional assays, such as ELISA, to measure its binding ability to other proteins like CD97 .

Recombinant CD55 is typically lyophilized from a filtered solution and can be reconstituted in sterile PBS. It is stable for up to 12 months when stored at -20 to -70°C and for shorter periods at 2-8°C after reconstitution .