CD52 Human
CD52 Molecule, Human Epididymis-Specific Protein 5, CD52 Antigen, Epididymal Secretory Protein E5, Cambridge Pathology 1 Antigen, CDW52, He5, Epididymis Secretory Sperm Binding Protein Li 171mP, CDW52 Antigen, CD52 Antigen, HEL-S-171mP, EDDM5, CDw52, HE5.
Greater than 95.0% as determined by SDS-PAGE.
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Description
CD52 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 251 amino acids (25-36 a.a.) and having a molecular mass of 28.2kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
CD52 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD52 Molecule, Human Epididymis-Specific Protein 5, CD52 Antigen, Epididymal Secretory Protein E5, Cambridge Pathology 1 Antigen, CDW52, He5, Epididymis Secretory Sperm Binding Protein Li 171mP, CDW52 Antigen, CD52 Antigen, HEL-S-171mP, EDDM5, CDw52, HE5.
GQNDTSQTSS PSLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE
SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.
Product Science Overview
CD52 is abundantly expressed on the surface of mature lymphocytes, including both T and B cells, as well as on monocytes, dendritic cells, eosinophils, and red blood cells . It is also found within the male reproductive tract, particularly on the surface of mature sperm cells . The molecule itself is highly negatively charged, which is thought to contribute to its function in preventing cell adhesion, allowing cells to move freely .
The primary function of CD52 is not entirely understood, but it is believed to play a role in the immune response. CD52 binds to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing sialic acid-binding lectin SIGLEC10 . This interaction may be involved in modulating immune cell activity and maintaining immune homeostasis.
CD52 is a critical target for therapeutic interventions, particularly in the treatment of hematological malignancies and in post-transplant immunosuppression . The monoclonal antibody alemtuzumab targets CD52 and is used in the treatment of chronic lymphocytic leukemia (CLL) and for preventing organ transplant rejection . Alemtuzumab works by binding to CD52 on the surface of lymphocytes, leading to their destruction through complement-mediated cell lysis .
In addition to its use in CLL, alemtuzumab has shown promise in the treatment of multiple sclerosis (MS). Clinical trials have demonstrated that alemtuzumab can reduce the relapse rate and accumulated disability in patients with relapsing-remitting MS .
Recombinant human CD52 proteins are produced using various expression systems, such as HEK293 cells . These recombinant proteins are used in research and therapeutic applications. They are typically biotinylated and purified to high standards, ensuring their suitability for use in various assays and clinical studies .
