CD48 Human
Sf9, Insect cells.
CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, SLAMF2, Signaling lymphocytic activation molecule 2, TCT.1.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD48 Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 203 amino acids (27-220a.a.) and having a molecular mass of 23.4kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
CD48 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, SLAMF2, Signaling lymphocytic activation molecule 2, TCT.1.
Sf9, Insect cells.
ADPQGHLVHM TVVSGSNVTL NISESLPENY KQLTWFYTFD QKIVEWDSRK SKYFESKFKG RVRLDPQSGA LYISKVQKED NSTYIMRVLK KTGNEQEWKI KLQVLDPVPK PVIKIEKIED MDDNCYLKLS CVIPGESVNY TWYGDKRPFP KELQNSVLET TLMPHNYSRC YTCQVSNSVS
SKNGTVCLSP PCTLARSHHH HHH.
Product Science Overview
The human CD48 cDNA encodes a 243 amino acid precursor, which includes a 26 amino acid signal sequence, a 194 amino acid mature protein, and a 23 amino acid C-terminal propeptide . The mature protein contains one Ig-like V-type domain and one Ig-like C2-type domain . CD48 is broadly expressed on hematopoietic cells but is excluded from quiescent long-term HSCs .
CD48 interacts with CD2 and CD244 (2B4), which are known ligands for CD48 . Through its interactions with CD244 on progenitor cells, CD48 influences HSC function by altering the bone marrow cytokine milieu, particularly interferon-gamma (IFNγ) . This interaction can either inhibit or stimulate IFNγ production from the target cell, depending on the context .
In CD48-null mice, the misregulation of cytokine signaling produces a more quiescent HSC, a disproportionate number of short-term progenitors, and hyperactivation of Pak1, leading to hematologic malignancies . CD48 plays a vital role as an environmental sensor for regulating HSC and progenitor cell numbers and inhibiting tumor development .
CD48 has been detected in the serum of patients with lymphoid leukemia and arthritis . It is also associated with diseases such as lymphoproliferative syndrome and Usher syndrome, type I . The recombinant form of CD48 is used in various research applications, including studies on immune responses and hematopoiesis.
Recombinant human CD48 is produced in a mouse myeloma cell line (NS0-derived) and is typically purified to a high degree of purity (>95%) by SDS-PAGE . It is often used in functional assays, such as ELISA, to study its binding ability and interactions with other proteins . The recombinant protein is available in both carrier-free and BSA-containing formulations, depending on the intended application .
