CD43 Human
CD43 Human Recombinant
Cat. No.
BT3392
Source
E.coli.
Synonyms
Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, SPN, CD43, leukosialin precursor, GPL115, LSN.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CD43 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (20-253a.a.) and having a molecular mass of 25.8kDa.
CD43 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CD43 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD43 is a crucial sialoglycoprotein found on the surface of various immune cells, including T lymphocytes, monocytes, granulocytes, thymocytes, and some B lymphocytes. It plays a role in T-cell activation as part of a ligand-receptor complex. During T-cell activation, CD43 is actively removed from the contact site between the T-cell and the antigen-presenting cell (APC), suggesting a negative regulatory role for CD43 in adaptive immune responses.
Description
Recombinant human CD43, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 257 amino acids (20-253a.a.). It has a molecular weight of 25.8 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The CD43 protein solution has a concentration of 1mg/ml and is supplied in 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, SPN, CD43, leukosialin precursor, GPL115, LSN.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSSTTAVQT PTSGEPLVST SEPLSSKMYT TSITSDPKAD STGDQTSALP PSTSINEGSP LWTSIGASTG SPLPEPTTYQ EVSIKMSSVP QETPHATSHP AVPITANSLG SHTVTGGTIT TNSPETSSRT SGAPVTTAAS SLETSRGTSG PPLTMATVSL ETSKGTSGPP VTMATDSLET STGTTGPPVT MTTGSLEPSS GASGPQVSSV KLSTMMSPTT STNASTVPFR NPDENSR.
Product Science Overview
Structure and Synthesis
In humans, CD43 is synthesized as a 400 amino acid precursor that includes:
- A 19 amino acid signal sequence
- A 234 amino acid extracellular region
- A 23 amino acid transmembrane domain
- A 124 amino acid cytoplasmic tail
The extracellular region of CD43 contains 25 O-linked glycosylation sites and one N-linked glycosylation site, contributing to its highly sialylated nature .
Functions
CD43 has diverse and context-specific functions:
Recombinant CD43
Applications
Storage and Stability
The recombinant protein is stable for 12 months when stored at -20 to -70°C as supplied. After reconstitution, it remains stable for one month at 2 to 8°C under sterile conditions and for three months at -20 to -70°C under sterile conditions .
CD43’s multifaceted roles in immune regulation make it a significant protein for research in immunology and cancer biology.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
