CD3D Human
CD3d Molecule, CD3d Antigen, Delta Polypeptide (TiT3 Complex), CD3d Molecule, Delta (CD3-TCR Complex), T-Cell Receptor T3 Delta Chain, T3D, T-Cell Surface Glycoprotein CD3 Delta Chain, CD3 Antigen, Delta Subunit, OKT3, Delta Chain, CD3d Antigen, CD3 Delta, CD3-DELTA, IMD19, CD3D.
Greater than 90.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
CD3D produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (22-105 a.a.) and fused to a 239 aa hIgG-His-tag at C-terminus containing a total of 323 amino acids and having a molecular mass of 36.5kDa.
CD3D shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
CD3D, alternatively known as Delta Polypeptide (TiT3 Complex), is a single-pass transmembrane protein classified as type 1. It forms a crucial part of the T-cell receptor-CD3 complex by associating with CD3-zeta, CD3-gamma, CD3-epsilon, and the T-cell receptor heterodimers (alpha/beta or gamma/delta). This complex is essential for T-cell activation. Upon activation of the T-cell receptor (TCR) by antigen-presenting cells (APCs), CD3 chains, including CD3D, CD3E, CD3G, and CD3Z, mediate the transmission of TCR-mediated signals across the cell membrane. CD3D plays a vital role in the adaptive immune response and contributes to the differentiation of thymocytes.
Produced in Sf9 Baculovirus cells, the recombinant CD3D protein consists of a single glycosylated polypeptide chain encompassing amino acids 22 to 105. It is fused at its C-terminus to a 239 amino acid hIgG-His-tag, resulting in a protein with a total of 323 amino acids and a molecular weight of 36.5 kDa. Under reducing conditions on SDS-PAGE, CD3D exhibits multiple bands ranging from 40 to 57 kDa. The protein undergoes purification using proprietary chromatographic techniques.
The CD3D protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer containing 10% glycerol and Phosphate-buffered saline (pH 7.4).
The purity of the CD3D protein is determined to be greater than 90.0% based on SDS-PAGE analysis.
CD3d Molecule, CD3d Antigen, Delta Polypeptide (TiT3 Complex), CD3d Molecule, Delta (CD3-TCR Complex), T-Cell Receptor T3 Delta Chain, T3D, T-Cell Surface Glycoprotein CD3 Delta Chain, CD3 Antigen, Delta Subunit, OKT3, Delta Chain, CD3d Antigen, CD3 Delta, CD3-DELTA, IMD19, CD3D.
FKIPIEELED RVFVNCNTSI TWVEGTVGTL LSDITRLDLG KRILDPRGIY RCNGTDIYKD KESTVQVHYR MCQSCVELDP ATVALEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH
Product Science Overview
CD3D is a single-pass type I membrane protein that, along with CD3-gamma, CD3-epsilon, and CD3-zeta, forms the TCR-CD3 complex . This complex is composed of a variable heterodimer (either TCRαβ or TCRγδ) responsible for ligand recognition and three invariant dimers (CD3γε, CD3δε, and ζζ/CD247) that participate in the assembly and surface expression of the TCR complex . The binding of the peptide-MHC complex by the TCR results in conformational changes in CD3, leading to the phosphorylation of tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the CD3 and ζζ/CD247 chains . This phosphorylation event triggers several signaling cascades that result in T-cell activation and the induction of a cellular immune response .
Recombinant human CD3D is produced using DNA sequences encoding the human CD3D extracellular domain. This recombinant protein is typically expressed in HEK293 cells and is used in various research applications . The recombinant human CD3D protein comprises 95 amino acids with a predicted molecular mass of 11 kDa. Due to glycosylation, it migrates as an approximately 20-23 kDa band in SDS-PAGE under reducing conditions .
Recombinant CD3D is used in research to study T-cell development, function, and signaling pathways. It is also utilized in the development of immunotherapies and diagnostic tools. Understanding the role of CD3D in the TCR complex and its impact on T-cell activation is crucial for developing targeted therapies for various immune-related disorders and diseases .
