CD320 Human
Sf9, Baculovirus cells.
CD320 antigen isoform 1, CD320, 8D6, 8D6A, TCBLR, FDC-SM-8D6, FDC-signaling molecule 8D6.
Greater than 90% as determined by SDS-PAGE.
Description
CD320 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 438 amino acids (31-229a.a.) and having a molecular mass of 47.4kDa. (Molecular size on SDS-PAGE will appear at approximately 57-70kDa). CD320 is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Cluster of Differentiation 320 (CD320), a member of the low-density lipoprotein receptor family, plays a crucial role in cobalamin (vitamin B12) uptake. It achieves this by binding to the transcobalamin II (TCN2)-cobalamin complex and facilitating its internalization through endocytosis. CD320 is characterized by two low-density lipoprotein receptor type A domains separated by a complement-like cysteine-rich region. Beyond its role in cobalamin uptake, CD320 also functions as a costimulator for B cell and follicular dendritic cells, contributing to their activation and response. Additionally, CD320 has been observed to enhance the proliferation of plasma cell (PC) precursors induced by Interleukin-10 (IL-10).
Recombinant human CD320, expressed in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. It consists of 438 amino acids (residues 31-229a.a.), resulting in a molecular weight of 47.4 kDa. However, on SDS-PAGE, it appears as a band at approximately 57-70 kDa due to glycosylation. The protein is engineered with a 239 amino acid human IgG-His tag at the C-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
The CD320 protein is supplied as a 0.5 mg/mL solution in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
For short-term storage (2-4 weeks), the protein can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein stability.
The purity of the CD320 protein is greater than 90% as determined by SDS-PAGE analysis.
CD320 antigen isoform 1, CD320, 8D6, 8D6A, TCBLR, FDC-SM-8D6, FDC-signaling molecule 8D6.
Sf9, Baculovirus cells.
LEAAASPLST PTSAQAAGPS SGSCPPTKFQ CRTSGLCVPL TWRCDRDLDC SDGSDEEECR IEPCTQKGQC PPPPGLPCPC TGVSDCSGGT DKKLRNCSRL ACLAGELRCT LSDDCIPLTW RCDGHPDCPD SSDELGCGTN EILPEGDATT MGPPVTLESV TSLRNATTMG PPVTLESVPS VGNATSSSAG DQSGSPTAYL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH.
Product Science Overview
CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer. It consists of a 199 amino acid extracellular domain with two cysteine-rich LDLR-A repeats, a 21 amino acid transmembrane segment, and a 32 amino acid cytoplasmic domain . Within the extracellular domain, human CD320 shares 52% amino acid sequence identity with mouse and rat CD320 .
The primary function of CD320 is to mediate the uptake of transcobalamin-bound cobalamin into cells. This process is essential for the proper functioning of various biological processes, including DNA synthesis and red blood cell formation.
Recombinant human CD320 is produced using a mouse myeloma cell line (NS0) and is available in various formulations for research purposes. The recombinant protein is typically purified to a high degree of purity (>90%) and is used in various applications, including functional assays and binding studies .
The recombinant human CD320 protein is often used in studies to understand its role in cobalamin metabolism and its potential implications in diseases related to vitamin B12 deficiency. It is also used in the development of therapeutic interventions targeting cobalamin uptake pathways.
Recombinant human CD320 is utilized in several research areas, including:
- Functional ELISA: To measure its binding ability to transcobalamin.
- Cell Culture: To study its role in cobalamin uptake and metabolism.
- Therapeutic Research: To develop potential treatments for conditions related to vitamin B12 deficiency.
