CD30 Human

CD30 Human Recombinant
Cat. No.
BT2682
Source
E.coli.
Synonyms
CD30, D1S166E, KI-1, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CD30 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 384 amino acids (19-379a.a.) and having a molecular mass of 40.8kDa. CD30 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD30, a member of the TNF-receptor superfamily, is also known as Tumor necrosis factor receptor superfamily member 8. This receptor is found on activated, but not resting, T and B cells. CD30 interacts with TRAF2 and TRAF5, which mediate signal transduction leading to the activation of NF-kappaB. CD30 positively regulates apoptosis and has been shown to limit autoreactive CD8 effector T cell proliferation, protecting the body from autoimmunity.
Description
Recombinant human CD30 is produced in E. coli. This single, non-glycosylated polypeptide chain contains 384 amino acids (19-379a.a.), has a molecular mass of 40.8 kDa, and is purified using proprietary chromatographic techniques. It contains a 23 amino acid His-tag at the N-terminus.
Physical Appearance
Sterile, colorless, and clear solution.
Formulation
The CD30 protein solution (1mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
CD30, D1S166E, KI-1, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSFPQDRPF EDTCHGNPSH YYDKAVRRCC YRCPMGLFPT QQCPQRPTDC RKQCEPDYYL DEADRCTACV TCSRDDLVEK TPCAWNSSRV CECRPGMFCS TSAVNSCARC FFHSVCPAGM IVKFPGTAQK NTVCEPASPG VSPACASPEN CKEPSSGTIP QAKPTPVSPA TSSASTMPVR GGTRLAQEAA SKLTRAPDSP SSVGRPSSDP GLSPTQPCPE GSGDCRKQCE PDYYLDEAGR CTACVSCSRD DLVEKTPCAW NSSRTCECRP GMICATSATN SCARCVPYPI CAAETVTKPQ DMAEKDTTFE APPLGTQPDC NPTPENGEAP ASTSPTQSLL VDSQASKTLP IPTSAPVALS STGK.

Product Science Overview

Structure and Function

Mature human CD30 consists of:

  • A 361 amino acid (aa) extracellular domain (ECD) with six cysteine-rich repeats
  • A 28 aa transmembrane segment
  • A 188 aa cytoplasmic domain

CD30 plays a crucial role in the regulation of immune responses. It is involved in the activation and proliferation of T cells and B cells, and it can influence the survival and apoptosis of these cells. The interaction of CD30 with its ligand, CD30L (CD30 Ligand/TNFSF8), can lead to the activation of various signaling pathways that are important for immune regulation .

Recombinant CD30

Recombinant human CD30 is produced using recombinant DNA technology, which involves inserting the gene encoding CD30 into a suitable expression system, such as a mouse myeloma cell line (NS0-derived human CD30/TNFRSF8 protein) . This allows for the production of large quantities of CD30 protein for research and therapeutic purposes.

Recombinant CD30 is used in various applications, including:

  • Research: Studying the role of CD30 in immune responses and its involvement in diseases such as Hodgkin’s lymphoma and other lymphomas.
  • Therapeutics: Developing targeted therapies for diseases associated with CD30 expression, such as antibody-drug conjugates (ADCs) that specifically target CD30-expressing cells.
Applications and Importance

The study of CD30 and its interactions with CD30L has significant implications for understanding and treating various immune-related diseases. By targeting CD30, researchers and clinicians can develop more effective therapies for conditions such as Hodgkin’s lymphoma, non-Hodgkin’s lymphomas, and other diseases involving abnormal T and B cell activation.

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