CD29 Antibody

CD29, Mouse Anti Human
Cat. No.
BT29161
Source
Synonyms
Integrin beta-1, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, CD29 antigen, ITGB1, CD29, FNRB, MDF2, VLAB, GPIIA, MSK12.
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Usage
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Description

Product Specs

Introduction
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix l
Formulation
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
CD29 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 - 1,000. Recommended starting dilution is 1:500.
Synonyms
Integrin beta-1, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, CD29 antigen, ITGB1, CD29, FNRB, MDF2, VLAB, GPIIA, MSK12.
Purification Method
CD29 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
Pk2D5AT.
Immunogen
Anti-human CD29 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CD29 amino acids 34-141 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Structure and Function

CD29 forms non-covalent associations with integrin α1-α6 chains to create very late antigen (VLA) molecules, specifically VLA-1 to VLA-6 . These integrins bind to several cell surface molecules, such as vascular cell adhesion molecule-1 (VCAM-1) and mucosal addressin cell adhesion molecule-1 (MadCAM-1), as well as extracellular matrix molecules like fibronectin . CD29 acts as a fibronectin receptor and plays a crucial role in various cell-cell and cell-matrix interactions .

Mouse Anti-Human CD29 Antibody

The mouse anti-human CD29 antibody is a monoclonal antibody that specifically binds to the human CD29 molecule. This antibody is used in various research and clinical applications, including flow cytometry, immunoprecipitation, and immunohistochemical staining . The antibody is typically conjugated with fluorochromes such as PE (phycoerythrin) for detection in flow cytometric analysis .

Applications
  1. Flow Cytometry: The mouse anti-human CD29 antibody is commonly used in flow cytometry to analyze the expression of CD29 on different cell types. The recommended usage is 5 µl per million cells in a 100 µl staining volume .
  2. Immunoprecipitation: This technique allows for the isolation and study of CD29 and its associated proteins from cell lysates .
  3. Immunohistochemistry: The antibody can be used to stain acetone-fixed frozen tissue sections, enabling the visualization of CD29 expression in various tissues .
Storage and Handling

The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light. It should not be frozen to maintain its stability and effectiveness .

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THE BIOTEK
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