CD27 Human, sf9

CD27 is a single-pass type I membrane protein. The human recombinant version of CD27 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 413 amino acids (21-191a.a.) and has a molecular mass of 46.4 kDa . The recombinant protein is fused with a 239 amino acids hIgG-His tag at the C-terminus and is purified using proprietary chromatographic techniques .

CD27 binds to its ligand, CD70, and this interaction is crucial for the regulation of B-cell activation and immunoglobulin synthesis . The CD27 receptor transduces signals that result in the activation of NF-kappaB and MAPK8/JNK pathways . These signaling pathways are essential for various cellular processes, including cell survival, proliferation, and differentiation.

Adaptor proteins TRAF2 and TRAF5 mediate the signaling process of CD27 . Additionally, CD27-binding protein (SIVA), a proapoptotic protein, can bind to the CD27 receptor and is believed to play a significant role in the apoptosis induced by CD27 .

The recombinant CD27 protein is widely used in laboratory research to study its role in immune regulation and its potential therapeutic applications. It is particularly valuable in research focused on T cell immunity, B-cell activation, and apoptosis .

The CD27 protein solution is typically formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol . For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to prevent multiple freeze-thaw cycles .