CD274 Mouse

The recombinant mouse CD274 protein is typically expressed in HEK293 cells. The extracellular domain of mouse CD274 (amino acids Met 1-Thr 238) is fused with a polyhistidine tag at the C-terminus for purification purposes . The secreted recombinant mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. Due to glycosylation, it migrates as an approximately 43-55 kDa band in SDS-PAGE under reducing conditions .

CD274 binds to its receptor, Programmed Cell Death Protein 1 (PD-1), leading to the inhibition of T-cell receptor-mediated lymphocyte proliferation and cytokine secretion . This interaction is crucial for maintaining peripheral immune tolerance and preventing autoimmunity. CD274 is also involved in the suppression of immune responses against tumors, making it a significant target for cancer immunotherapy .

CD274 is often overexpressed in various tumors, where it contributes to immune evasion by inhibiting the activity of T-cells. This overexpression is associated with poor prognosis and higher malignancy grades in cancer patients . Therapeutic antibodies targeting the PD-1/PD-L1 pathway have shown promise in reactivating T-cell activity and enhancing antitumor immunity .

Recombinant mouse CD274 is widely used in research to study its role in immune regulation and cancer. It is also employed in the development of therapeutic antibodies and in functional assays to investigate the binding interactions between PD-L1 and PD-1 .

Recombinant mouse CD274 is typically provided as a lyophilized powder and is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles .