CD244 Human, Sf9
Sf9, Insect cells.
Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NAIL, NK cell type I receptor protein 2B4, NKR2B4, h2B4, SLAM family member 4, SLAMF4, Signaling lymphocytic activation molecule 4, CD244, 2B4.
Greater than 90% as determined by SDS-PAGE.
Description
CD244 Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 448 amino acids (19-224a.a.) and having a molecular mass of 50.2kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). CD244 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NAIL, NK cell type I receptor protein 2B4, NKR2B4, h2B4, SLAM family member 4, SLAMF4, Signaling lymphocytic activation molecule 4, CD244, 2B4.
Sf9, Insect cells.
ADLGKGCQGS ADHVVSISGV PLQLQPNSIQ TKVDSIAWKK LLPSQNGFHH ILKWENGSLP SNTSNDRFSF IVKNLSLLIK AAQQQDSGLY CLEVTSISGK VQTATFQVFV FDKVEKPRLQ GQGKILDRGR CQVALSCLVS RDGNVSYAWY RGSKLIQTAG NLTYLDEEVD INGTHTYTCN VSNPVSWESH TLNLTQDCQN AHQEFRFWPL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH.
Product Science Overview
CD244 contains two Ig-like (immunoglobulin-like) domains and is involved in mediating non-major histocompatibility complex (MHC) restricted killing . The interaction between NK cells and target cells via the CD244 receptor modulates NK-cell cytolytic activity . Additionally, CD244 molecules function in eosinophil adhesion and chemotaxis and play a role in the pathophysiology of allergic rhinitis (AR) .
CD244 Human Recombinant produced in Sf9 insect cells is a single, glycosylated polypeptide chain containing 448 amino acids (19-224a.a.) and has a molecular mass of 50.2 kDa . The recombinant protein is expressed with a 239 amino acids hIgG-His tag at the C-terminus and is purified using proprietary chromatographic techniques . The molecular size on SDS-PAGE appears at approximately 50-70 kDa .
CD244 binds to the ligand CD48 on adjacent cells and transmits stimulatory or inhibitory signals that regulate immune function . This interaction is essential for the expansion and activation of NK cells, and it regulates various immune responses such as cytotoxicity, cytokine production, and intercellular interactions . Abnormal interactions between immune cells involving CD244 contribute to the onset and progression of autoimmune diseases, infectious diseases, and cancers .
The CD244 protein solution is formulated with Phosphate Buffered Saline (pH 7.4) and 10% glycerol . It is recommended to store the solution at 4°C if used within 2-4 weeks, or at -20°C for longer periods. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised to avoid multiple freeze-thaw cycles .
