KEL Mouse
kell blood group antigen, kell blood group glycoprotein, Kell blood group, Kell blood group glycoprotein homolog, KEL, Kell, CD238 antigen, CD238, ECE3, Kell blood group-metalloendopeptidase, Kell blood group-metalloendopeptidase.
Greater than 90.0% as determined by SDS-PAGE.
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Description
KEL Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 674 amino acids (49-713 aa) and having a molecular mass of 76.3kDa.
KEL is fused to a 9 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
kell blood group antigen, kell blood group glycoprotein, Kell blood group, Kell blood group glycoprotein homolog, KEL, Kell, CD238 antigen, CD238, ECE3, Kell blood group-metalloendopeptidase, Kell blood group-metalloendopeptidase.
ADPIFRNCGP CPCETPVCME LLDHYLASGN RSVAPCTDFF SFACEKANGT SDSFQALTEE NKSRLWRLLE APGSWHLGSG EEKAFQFYNS CMDTDAIEAS GSGPLIQIIE ELGGWNITGN WTSLDFNQNL RLLMSQYGHF PFFRAYLRPH PAPPHTPIIQ IDQPEFDILL QQEQEQKVYA QILREYVTYL NRLGTLLGSN PQEAQQHASW SIVFTSRLFQ FLRPQQQQQA QDKLFHVVTI DELQEMAPAI DWLSCLQAIF TPMSLNSSQT LVVHDLDYLR NMSQLVEEGL LNHRESIQSY MILGLVDTLS PALDTKFQEA RRELIQELRK LKERPPLPAY PRWMKCVEQT GAFFEPTLAA LFVREAFGPS IQSAAMELFA EIKDAVIIRL KKLSWISEET QKEALNKLAQ LQVEMGAPKR AVKPDIATQE YNDIQLGPSF LQSFLSCVRS LRARNVQSFL QPFPYHRWQK SPWEVNAYYS ISDHMVVFPA GLLQPPFFHP GYPRAVNFGA AGSIMAHELL HIFYQLLLPG GCPACDTHVL QEALLCLERH YAAFPLPSIS SFNGSHTLLE NAADIGGVAI AFQAYSKRIV EHTGELTLPN LDLSPYQLFF RSYAQVMCRG LSSQDPQDPH SPPSLRVHGP LSNTPDFAKH FHCPRGTLLN PSARCKLWHH HHHH.
Product Science Overview
Kell Metallo-Endopeptidase, also known as KEL or CD238, is a zinc-dependent endopeptidase that belongs to the neprilysin (NEP) family of proteins. This family includes other important enzymes such as endothelin-converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE, and several NEP-like proteins . The Kell protein is crucial in the production of bioactive endothelin-3 (ET-3) and plays a significant role in various physiological processes.
The recombinant mouse Kell protein is produced in Sf9 Baculovirus cells and is a single, glycosylated polypeptide chain containing 674 amino acids (49-713 aa) with a molecular mass of approximately 76.3 kDa . It is fused to a 9 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques . The protein is typically supplied as a sterile, filtered colorless solution containing 10% glycerol and phosphate-buffered saline (pH 7.4) .
Kell Metallo-Endopeptidase cleaves big endothelin-3 (ET-3) at the Trp21-Ile22 bond, yielding ET-3 . It also cleaves big endothelin-1 (ET-1) and big endothelin-2 (ET-2) at the Trp21-Val22 bond, although to a much lesser extent . The enzyme’s activity is measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp)-OH . The specific activity of the recombinant mouse Kell protein is greater than 0.2 pmol/min/µg under the described conditions .
The Kell protein is part of the Kell blood group system, which is one of the major blood group systems in humans. It is involved in various physiological processes, including the regulation of blood pressure and electrolyte balance . Mice homozygous for a null allele of the Kell gene exhibit decreased heart rate, altered hematological parameters, and mild motor deficits . These findings highlight the importance of the Kell protein in maintaining normal physiological functions.
The recombinant mouse Kell protein should be stored at 4°C if it will be used within 2-4 weeks. For longer periods, it should be stored frozen at -20°C, and it is recommended to add a carrier protein (0.1% HSA or BSA) to prevent degradation . Avoiding multiple freeze-thaw cycles is crucial to maintain the protein’s stability and activity .
