CD209 Human
Sf9, Baculovirus cells.
CD209 antigen, CD209 antigen isoform 1, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, DC-SIGN, DC-SIGN1, CD209, CLEC4L, CDSIGN
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD209 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 587 amino acids (60-404 a.a) and having a molecular mass of 66.5kDa.
CD209 is fused to a 239 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
CD209, a member of the C-type lectin family, is a pathogen-recognition receptor found on the surface of immature dendritic cells (DCs). It plays a crucial role in initiating the primary immune response. CD209 exhibits high affinity for the ICAM3 molecule and binds to various microorganisms by recognizing high-mannose containing glycoproteins on their envelopes. Notably, it acts as a receptor for several viruses, including HIV and Hepatitis C. In conjunction with other C-type lectins, CD209 contributes to the recognition of tumors by dendritic cells. Furthermore, it initiates natural immunity by modulating toll-like receptors.
Recombinant human CD209, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 587 amino acids (60-404 a.a). It has a molecular mass of 66.5 kDa. The CD209 protein is fused to a 239 amino acid hIgG-His-Tag at the C-terminus and is purified using proprietary chromatographic techniques.
Sterile, colorless solution.
The CD209 solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 10% glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
The purity of the CD209 protein is greater than 90.0% as determined by SDS-PAGE analysis.
CD209 antigen, CD209 antigen isoform 1, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, DC-SIGN, DC-SIGN1, CD209, CLEC4L, CDSIGN
Sf9, Baculovirus cells.
ADPVSKVPSS ISQEQSRQDA IYQNLTQLKA AVGELSEKSK LQEIYQELTQ LKAAVGELPE KSKLQEIYQE LTRLKAAVGE LPEKSKLQEI YQELTWLKAA VGELPEKSKM QEIYQELTRL KAAVGELPEK SKQQEIYQEL TRLKAAVGEL PEKSKQQEIY QELTRLKAAV GELPEKSKQQ EIYQELTQLK AAVERLCHPC PWEWTFFQGN CYFMSNSQRN WHDSITACKE VGAQLVVIKS AEEQNFLQLQ SSRSNRFTWM GLSDLNQEGT WQWVDGSPLL PSFKQYWNRG EPNNVGEEDC AEFSGNGWND DKCNLAKFWI CKKSAASCSR DEEQFLSPAP ATPNPPPAVE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH
Product Science Overview
The canonical isoform of CD209 is a 46 kDa protein composed of 404 amino acids . It features an extracellular region that contains a calcium-dependent carbohydrate-binding lectin domain. This domain is essential for its function in recognizing and binding to specific carbohydrate structures on the surface of various pathogens, including viruses, bacteria, and fungi .
CD209 is involved in the capture and internalization of pathogens, facilitating their presentation to T cells and initiating an immune response. It specifically binds to high-mannose-type carbohydrates, which are commonly found on the surface of many pathogens .
CD209 has been implicated in several infectious diseases. For instance, it serves as a receptor for the Dengue virus and the Human Immunodeficiency Virus Type 1 (HIV-1) . By binding to these viruses, CD209 facilitates their entry into dendritic cells, which can lead to the spread of infection within the host .
Recombinant CD209 proteins are produced using various expression systems, such as Chinese Hamster Ovary (CHO) cells . These recombinant proteins are used in research to study the function of CD209 and its interactions with pathogens. They are also employed in the development of therapeutic strategies aimed at modulating the immune response.
Recombinant CD209 proteins are typically purified to high levels of purity (>95%) and are tested for endotoxin levels to ensure their suitability for experimental use . They are available in different formulations, including carrier-free versions, which do not contain Bovine Serum Albumin (BSA), making them suitable for applications where the presence of BSA could interfere with experimental outcomes .
Recombinant CD209 proteins are valuable tools in immunology and infectious disease research. They are used to study the mechanisms of pathogen recognition and uptake by dendritic cells. Additionally, they are employed in assays to investigate the binding interactions between CD209 and various ligands, including viral glycoproteins and bacterial polysaccharides .
