CD1a Antibody
CD1a (leu6, T6), Mouse Anti-Human
Cat. No.
BT28680
Source
Synonyms
T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, R4, T6; CD1, FCB6, HTA1.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
CD1a is a transmembrane glycoprotein that belongs to the MHC protein family. It forms heterodimers with beta-2-microglobulin and plays a crucial role in presenting lipid and glycolipid antigens to T cells. These antigens can be derived from both the self and microbes. The human genome contains five CD1 family genes located on chromosome 1. Each CD1 family member is believed to have distinct cellular localization and specificity for lipid ligands. This particular gene encodes a protein that is found on the plasma membrane and in recycling vesicles of the early endocytic system. While alternatively spliced transcript variants exist, their biological significance remains to be determined.
Formulation
Supplied as a 1 mg/ml solution in PBS buffer after reconstitution.
Storage Procedures
Store the lyophilized product at 4°C. After reconstitution, the antibody is stable for one month at 4°C. For long-term storage, aliquot the reconstituted antibody and store at -20°C.
Solubility
Reconstitute the antibody by adding the appropriate amount of sterile H2O. Gently mix the solution, ensuring the contents are dissolved by washing the sides of the vial. Allow 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for staining applications. The recommended concentration for staining is 10 µl per 10^6 cells. It specifically recognizes human thymocytes.
Synonyms
T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, R4, T6; CD1, FCB6, HTA1.
Purification Method
Ion exchange column.
Type
Mouse Anti Human Monoclonal.
Clone
hCD1a.
Immunogen
Purified human PBL T cells.
Ig Subclass
Mouse IgG1.
Product Science Overview
Introduction
CD1a, also known as Leu-6 or T6, is a 40 kDa type I membrane glycoprotein that belongs to the immunoglobulin superfamily. It shares structural and functional similarities with MHC class I molecules and is associated with β2-microglobulin . CD1a plays a crucial role in antigen presentation by binding lipid and glycolipid antigens and presenting them to T cell receptors on natural killer T cells .
Expression and Function
CD1a is predominantly expressed on Langerhans cells, dendritic cells, and cortical thymocytes . Its primary function is to present lipid antigens to T cells, which is essential for the immune response. This antigen presentation is vital for the activation of T cells and the subsequent immune response against pathogens .
Mouse Anti-Human CD1a Antibodies
Applications
- Flow Cytometry: Mouse anti-human CD1a antibodies are widely used in flow cytometry to identify and quantify CD1a-expressing cells. This application is particularly useful in immunophenotyping and studying the immune system .
- Immunohistochemistry: These antibodies are also employed in immunohistochemistry to detect CD1a expression in tissue samples. This technique is valuable for studying the distribution and localization of CD1a-expressing cells in various tissues .
- Immunofluorescence: In immunofluorescence assays, mouse anti-human CD1a antibodies are used to visualize CD1a expression in cells and tissues. This application helps in understanding the cellular and subcellular localization of CD1a .
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