CD19 Human
CD19 Molecule, B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12, Differentiation Antigen CD19, CD19 Antigen, B-Lymphocyte Antigen CD19, CVID3, B4.
Greater than 85.0% as determined by SDS-PAGE.
Description
CD19 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 510 amino acids (21-291a.a.) and having a molecular mass of 57.0kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
CD19 is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD19, also referred to as B-Lymphocyte Surface Antigen B4, is a member of the immunoglobulin superfamily and is found on the surface of almost all B-lymphocytes, excluding fully matured plasma cells. Functioning as a signal-transducing receptor, CD19 plays a crucial role in regulating both B cell differentiation and proliferation. It acts as an adaptor protein, recruiting cytoplasmic signaling proteins to the cell membrane. Working in conjunction with the CD19/CD21 complex, it lowers the activation threshold of B cell receptor signaling pathways. Given its presence on most B cells, CD19 serves as a valuable biomarker in diagnosing lymphoma, understanding B lymphocyte development, and as a target for immunotherapies targeting leukemia.
CD19, produced using Sf9 Baculovirus cells, is a single-chain polypeptide with glycosylation. It consists of 510 amino acids (21-291a.a.) and has a molecular weight of 57.0kDa. Note: On SDS-PAGE, the apparent molecular size will be approximately 50-70kDa. This CD19 protein is expressed with a 239 amino acid hIgG-His tag at its C-Terminus and is purified using proprietary chromatographic techniques.
The provided CD19 protein solution has a concentration of 0.25mg/ml. It is prepared in a solution containing Phosphate Buffered Saline at a pH of 7.4 and 10% glycerol.
Analysis by SDS-PAGE indicates that the purity of this CD19 protein is greater than 85.0%.
CD19 Molecule, B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12, Differentiation Antigen CD19, CD19 Antigen, B-Lymphocyte Antigen CD19, CVID3, B4.
EEPLVVKVEE GDNAVLQCLK GTSDGPTQQL TWSRESPLKP FLKLSLGLPG LGIHMRPLAI WLFIFNVSQQ MGGFYLCQPG PPSEKAWQPG WTVNVEGSGE LFRWNVSDLG GLGCGLKNRS SEGPSSPSGK LMSPKLYVWA KDRPEIWEGE PPCLPPRDSL NQSLSQDLTM APGSTLWLSC GVPPDSVSRG PLSWTHVHPK GPKSLLSLEL KDDRPARDMW VMETGLLLPR ATAQDAGKYY CHRGNLTMSF HLEITARPVL WHWLLRTGGW KLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH
Product Science Overview
CD19 is a 95 kDa transmembrane glycoprotein composed of several domains:
- Extracellular Domain (ECD): This domain consists of 272 amino acids and contains two immunoglobulin-like domains .
- Transmembrane Segment: A 22 amino acid segment that anchors the protein to the cell membrane .
- Cytoplasmic Domain: Comprising 243 amino acids, this domain is involved in intracellular signaling .
The primary function of CD19 is to lower the threshold for B cell receptor (BCR) signaling, thereby enhancing the sensitivity of B cells to antigens. This modulation is critical for the activation and differentiation of B cells, which are essential components of the adaptive immune system .
CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains . It is also expressed on myeloid leukemia cells, particularly those of monocytic lineage . Due to its consistent expression on B cells, CD19 serves as a valuable biomarker for both normal and neoplastic B cells . It is also used in the diagnosis and treatment of B cell-related diseases, including various forms of lymphoma and leukemia .
Recombinant CD19 proteins are produced using recombinant DNA technology, which involves inserting the CD19 gene into an expression system, such as bacteria, yeast, or mammalian cells. This allows for the large-scale production of CD19 proteins, which can be used for research, diagnostic, and therapeutic purposes.
For example, the recombinant human CD19 protein consists of 283 amino acids and has a predicted molecular mass of 31.6 kDa. However, due to glycosylation, it migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions .
Recombinant CD19 proteins have several applications:
- Research: Used to study B cell development, activation, and signaling pathways.
- Diagnostics: Employed in flow cytometry and immunohistochemistry to identify and quantify B cells in clinical samples.
- Therapeutics: Utilized in the development of targeted therapies, such as chimeric antigen receptor (CAR) T-cell therapy, which involves engineering T cells to express a receptor that targets CD19 on B cells.
