CD18 Human
CD18 Human Recombinant
Cat. No.
BT1650
Source
Sf9, Baculovirus cells.
Synonyms
Integrin Subunit Beta 2, Integrin, Beta 2 (Complement Component 3 Receptor 3 And 4 Subunit), Complement Component 3 Receptor 3 And 4 Subunit, CD18, MFI7, Integrin, Beta 2 (Antigen CD18 (P95), Lymphocyte Function-Associated Antigen 1; Macrophage Antigen 1 (Mac-1) Beta Subunit), Cell Surface Adhesion Glycoprotein (LFA-1/CR3/P150,959 Beta Subunit Precursor), Cell Surface Adhesion Glycoproteins LFA-1/CR3/P150,95 Subunit Beta, Leukocyte-Associated Antigens CD18/11A, CD18/11B, CD18/11C, Leukocyte Cell Adhesion Molecule CD18, Complement Receptor C3 Beta-Subunit, Complement Receptor C3 Subunit Beta, Integrin Beta Chain, Beta 2, CD18 Antigen, LFA-1, MAC-1, LCAMB, MF17, LAD, CD18.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CD18 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 686 amino acids (23-700 a.a.) and having a molecular mass of 75.9kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions).
CD18 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
CD18 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD18, also known as ITGB2 or integrin beta-2, acts as a receptor for ICAM1, ICAM2, ICAM3, and ICAM4. It is believed to be part of a larger family of cell surface molecules that includes the fibronectin binding protein. Additionally, CD18 functions as a complement receptor type 3 (CR3). During lung injury, CD18 plays a role in neutrophil transmigration by activating PTK2B/PYK2.
Description
Recombinant human CD18, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 686 amino acids (23-700 a.a.). It has a molecular mass of 75.9kDa and migrates at 70-100kDa on SDS-PAGE under reducing conditions. This protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The CD18 protein solution is provided at a concentration of 0.25mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For longer storage periods, it is recommended to freeze the product at -20°C. To further enhance long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of the CD18 protein is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
Integrin Subunit Beta 2, Integrin, Beta 2 (Complement Component 3 Receptor 3 And 4 Subunit), Complement Component 3 Receptor 3 And 4 Subunit, CD18, MFI7, Integrin, Beta 2 (Antigen CD18 (P95), Lymphocyte Function-Associated Antigen 1; Macrophage Antigen 1 (Mac-1) Beta Subunit), Cell Surface Adhesion Glycoprotein (LFA-1/CR3/P150,959 Beta Subunit Precursor), Cell Surface Adhesion Glycoproteins LFA-1/CR3/P150,95 Subunit Beta, Leukocyte-Associated Antigens CD18/11A, CD18/11B, CD18/11C, Leukocyte Cell Adhesion Molecule CD18, Complement Receptor C3 Beta-Subunit, Complement Receptor C3 Subunit Beta, Integrin Beta Chain, Beta 2, CD18 Antigen, LFA-1, MAC-1, LCAMB, MF17, LAD, CD18.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
QECTKFKVSS CRECIESGPG CTWCQKLNFT GPGDPDSIRC DTRPQLLMRG CAADDIMDPT SLAETQEDHN GGQKQLSPQK VTLYLRPGQA AAFNVTFRRA KGYPIDLYYL MDLSYSMLDD LRNVKKLGGD LLRALNEITE SGRIGFGSFV DKTVLPFVNT HPDKLRNPCP NKEKECQPPF AFRHVLKLTN NSNQFQTEVG KQLISGNLDA PEGGLDAMMQ VAACPEEIGW RNVTRLLVFA TDDGFHFAGD GKLGAILTPN DGRCHLEDNL YKRSNEFDYP SVGQLAHKLA ENNIQPIFAV TSRMVKTYEK LTEIIPKSAV GELSEDSSNV VQLIKNAYNK LSSRVFLDHN ALPDTLKVTY DSFCSNGVTH RNQPRGDCDG VQINVPITFQ VKVTATECIQ EQSFVIRALG FTDIVTVQVL PQCECRCRDQ SRDRSLCHGK GFLECGICRC DTGYIGKNCE CQTQGRSSQE LEGSCRKDNN SIICSGLGDC VCGQCLCHTS DVPGKLIYGQ YCECDTINCE RYNGQVCGGP GRGLCFCGKC RCHPGFEGSA CQCERTTEGC LNPRRVECSG RGRCRCNVCE CHSGYQLPLC QECPGCPSPC GKYISCAECL KFEKGPFGKN CSAACPGLQL SNNPVKGRTC KERDSEGCWV AYTLEQQDGM DRYLIYVDES RECVAGPNLE HHHHHH
Product Science Overview
Structure and Function
CD18 forms heterodimers with different alpha chains, such as CD11a, CD11b, and CD11c, to create various integrin complexes like LFA-1 (Lymphocyte Function-associated Antigen 1), Mac-1 (Macrophage-1 Antigen), and CR4 (Complement Receptor 4) . These complexes are essential for leukocyte adhesion and migration, which are critical for immune responses.
Clinical Significance
Recombinant CD18
Recombinant CD18 is produced using recombinant DNA technology, which involves inserting the human ITGB2 gene into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. This recombinant protein can be used for various research applications, including studying the molecular mechanisms of cell adhesion, developing therapeutic antibodies, and investigating the role of integrins in diseases .
Applications in Research
Recombinant CD18 is widely used in immunological research to study leukocyte behavior, cell signaling pathways, and the development of new therapeutic strategies for immune-related diseases. It is also used in flow cytometry and other assays to investigate the expression and function of integrins on the cell surface .
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