SELPLG Human
HEK293 Cells.
Cutaneous Lymphocyte-Associated Associated Antigen, Selectin P Ligand, PSGL-1, CD162 Antigen, P-Selectin Glycoprotein Ligand 1, CLA.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
SELPLG Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 496 amino acids (42-295 a.a) and having a molecular mass of 53.4kDa.
SELPLG is fused to a 239 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
The SELPLG glycoprotein serves as a high-affinity counter-receptor for selectin molecules (P, E, and L), which are found on stimulated T lymphocytes and myeloid cells. It plays a crucial role in leukocyte trafficking during inflammation by binding leukocytes to activated platelets or endothelia expressing selectins. SELPLG requires two post-translational modifications for high-affinity binding: tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans. Variations and irregular expression of SELPLG have been associated with abnormalities in the innate and adaptive immune responses. Alternative splicing results in different transcript variants.
Recombinant Human SELPLG, produced in HEK293 cells, is a single, glycosylated polypeptide chain comprising 496 amino acids (42-295 a.a.). It has a molecular weight of 53.4 kDa. The SELPLG is fused to a 239 amino acid hIgG-His-Tag at its C-terminus and is purified using proprietary chromatographic methods.
Sterile filtered, colorless solution.
The SELPLG solution (concentration: 1 mg/ml) is prepared in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Cutaneous Lymphocyte-Associated Associated Antigen, Selectin P Ligand, PSGL-1, CD162 Antigen, P-Selectin Glycoprotein Ligand 1, CLA.
HEK293 Cells.
DGSQATEYEY LDYDFLPETE PPEMLRNSTD TTPLTGPGTP ESTTVEPAAR RSTGLDAGGA VTELTTELAN MGNLSTDSAA MEIQTTQPAA TEAQTTPLAA TEAQTTRLTA TEAQTTPLAA TEAQTTPPAA TEAQTTQPTG LEAQTTAPAA MEAQTTAPAA MEAQTTPPAA MEAQTTQTTA MEAQTTAPEA TEAQTTQPTA TEAQTTPLAA MEALSTEPSA TEALSMEPTT KRGLFIPFSV SSVTHKGIPM AASNLSVLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH
Product Science Overview
PSGL-1 is expressed on the surface of various hematopoietic cells, including neutrophils, eosinophils, lymphocytes, and monocytes . It plays a crucial role in mediating the tethering and adhesion of these cells to the endothelium, facilitating their migration to sites of inflammation or injury .
The interaction between PSGL-1 and P-selectin is highly specific and involves several structural features:
- Glycosylation: PSGL-1 is heavily glycosylated, with a core 2 O-glycan expressing the unique tetrasaccharide sialyl-Lewis x (sLe X), which is essential for high-affinity binding to P-selectin .
- Tyrosine Sulfation: The presence of tyrosine sulfate residues at positions 46, 48, and 51 significantly enhances the binding affinity of PSGL-1 to P-selectin .
- Fucose and Sialic Acid: These sugar residues also contribute to the high-affinity interaction between PSGL-1 and P-selectin .
While PSGL-1 is essential for normal immune function, its interaction with P-selectin can also contribute to various pathological conditions:
- Thromboinflammation: The P-selectin/PSGL-1 pathway is implicated in thromboinflammatory processes, where both inflammatory and thrombotic pathways are activated simultaneously, contributing to diseases such as atherosclerosis, thrombosis, and metabolic syndrome .
- Cardiovascular Diseases: Sustained expression of P-selectin and its interaction with PSGL-1 can lead to cardiovascular diseases, including stroke and heart attacks .
- Autoimmune Diseases and Cancer: Dysregulation of the P-selectin/PSGL-1 pathway is also associated with autoimmune diseases and cancer .
