CD160 Human
Sf9, Baculovirus cells.
CD160 Molecule, CD160 Antigen, Natural Killer Cell Receptor BY55, BY55, Natural Killer Cell Receptor, Immunoglobulin Superfamily Member , CD160 Transmembrane Isoform, CD160-Delta Ig, NK28, NK1, CD160.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD160 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 142 amino acids (27-159a.a.) and having a molecular mass of 15.9kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). CD160 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The CD160 antigen, also known as CD160, functions as an activating receptor on human natural killer (NK) cells and is present on specific subsets of T cells. This antigen plays a critical role as a tumor-specific entity, particularly in chronic lymphocytic leukemia (CLL), where it mediates the activation of these cells. This characteristic makes CD160 a potential target for therapeutic interventions and the monitoring of minimal residual disease. Research has identified CD160 as a coinhibitory molecule on T cells that interacts with the herpesvirus entry mediator (HVEM) found on antigen-presenting cells. This interaction generates a potent inhibitory signal directed at CD4(+) T cells. The expression of CD160 is predominantly observed on peripheral blood NK cells and CD8 T lymphocytes, both of which exhibit cytolytic effector functions. Additionally, CD160 is present on all intestinal intraepithelial lymphocytes and demonstrates a broad binding capacity to both classical and nonclassical MHC class I molecules.
Recombinant human CD160, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 142 amino acids (specifically, residues 27 to 159a.a.) and has a molecular weight of 15.9 kDa. However, it's important to note that on SDS-PAGE analysis, the apparent molecular size will be approximately 18-28 kDa. This discrepancy is attributed to glycosylation. The CD160 protein is engineered to include a 6-amino acid His tag at its C-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
The CD160 protein solution is provided at a concentration of 0.25 mg/ml. The solution is buffered with Phosphate Buffered Saline (PBS) at a pH of 7.4 and contains 10% glycerol to enhance stability.
The purity of the CD160 protein is greater than 90%, as determined by SDS-PAGE analysis.
CD160 Molecule, CD160 Antigen, Natural Killer Cell Receptor BY55, BY55, Natural Killer Cell Receptor, Immunoglobulin Superfamily Member , CD160 Transmembrane Isoform, CD160-Delta Ig, NK28, NK1, CD160.
Sf9, Baculovirus cells.
ADLINITSSA SQEGTRLNLI CTVWHKKEEA EGFVVFLCKD RSGDCSPETS LKQLRLKRDP GIDGVGEISS QLMFTISQVT PLHSGTYQCC ARSQKSGIRL QGHFFSILFT ETGNYTVTGL KQRQHLEFSH NEGTLSHHHH HH.
Product Science Overview
CD160 is a glycosylphosphatidylinositol (GPI)-anchored protein, meaning it is attached to the cell membrane via a GPI anchor . The protein is synthesized as a preproprotein that is 181 amino acids in length. This precursor contains a 26 amino acid signal sequence, a 133 amino acid mature molecule with a V-type Ig-like domain, and a 22 amino acid prosegment that is cleaved to generate the GPI linkage .
The expression of CD160 is restricted to specific immune cells, particularly those involved in the body’s innate and adaptive immune responses. It is notably present on CD56dim CD16+ NK cells, which are a subset of NK cells with potent cytotoxic activity .
CD160 plays a crucial role in the immune system. It is involved in the regulation of immune responses, particularly in the context of cytotoxic T cells and NK cells. The protein binds to MHC class I molecules, which are essential for the presentation of antigens to T cells. This interaction is critical for the activation and regulation of immune responses against infected or malignant cells .
Recombinant CD160 (Human) is a form of the protein that is produced using recombinant DNA technology. This involves inserting the gene encoding CD160 into a suitable expression system, such as HEK 293 cells, to produce the protein in vitro. The recombinant protein is often tagged with a His tag to facilitate purification and is used in various research applications, including studies on immune cell function and interactions .
