CCDC69 Human

Coiled-Coil Domain Containing 69 Human Recombinant
Cat. No.
BT11749
Source
Escherichia Coli.
Synonyms
Coiled-coil domain-containing protein 69, CCDC69.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CCDC69 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 319 amino acids (1-296 a.a) and having a molecular mass of 37.2kDa.
CCDC69 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CCDC69, also known as Coiled-Coil Domain Containing 69, is a protein-coding gene. MTUS1 is a significant paralog of this gene.
Description
Recombinant human CCDC69 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 319 amino acids (with amino acids 1-296 present) and has a molecular weight of 37.2 kDa. A 23 amino acid His-tag is fused to the N-terminus of CCDC69. Purification is carried out using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The provided CCDC69 protein solution has a concentration of 0.25 mg/ml. The solution contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Coiled-coil domain-containing protein 69, CCDC69.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGCRHSR LSSCKPPKKK RQEPEPEQPP RPEPHELGPL NGDTAITVQL CASEEAERHQ KDITRILQQH EEEKKKWAQQ VEKERELELR DRLDEQQRVL EGKNEEALQV LRASYEQEKE ALTHSFREAS STQQETIDRL TSQLEAFQAK MKRVEESILS RNYKKHIQDY GSPSQFWEQE LESLHFVIEM KNERIHELDR RLILMETVKE KNLILEEKIT TLQQENEDLH VRSRNQVVLS RQLSEDLLLT REALEKEVQL RRQLQQEKEE LLYRVLGANA SPAFPLAPVT PTEVSFLAT.

Product Science Overview

Gene and Protein Information
  • Gene Name: CCDC69
  • Protein Name: Coiled-Coil Domain Containing 69
  • Gene ID: 26112 (NCBI)
  • UniProt ID: A6NI79
  • Chromosomal Location: Chromosome 5

The CCDC69 gene is involved in encoding a protein that is predicted to enable microtubule binding activity. This protein is crucial for the proper assembly of the spindle midzone, a structure that forms during cell division and is essential for the segregation of chromosomes.

Functional Role

CCDC69 is believed to act as a scaffold protein, regulating the recruitment and assembly of spindle midzone components. It is required for the localization of key proteins such as Aurora B kinase (AURKB) and Polo-like kinase 1 (PLK1) to the spindle midzone . These proteins are essential for the proper progression of mitosis, ensuring accurate chromosome segregation and cell division.

Expression and Localization

The CCDC69 protein is primarily localized to the spindle midzone during cell division. It has been observed to physically interact with Aurora B kinase, a component of the chromosomal passenger complex (CPC), and other proteins involved in spindle midzone assembly . This interaction is crucial for the stability and function of the spindle midzone.

Recombinant Protein

Human recombinant CCDC69 is produced using various expression systems, such as E. coli. The recombinant protein is often used in research to study its function and interactions with other proteins. It is typically purified and supplied in a liquid form, with a concentration of ≥5.0 mg/mL .

Associated Diseases

Mutations or dysregulation of the CCDC69 gene have been associated with certain diseases, including:

  • Deafness, Autosomal Recessive 42: A form of hereditary hearing loss.
  • Familial Apolipoprotein C-II Deficiency: A rare genetic disorder affecting lipid metabolism .
Research Applications

The recombinant CCDC69 protein is used in various research applications, including:

  • Blocking Assays: To study protein-protein interactions.
  • Immunohistochemistry (IHC) and Immunocytochemistry (ICC): To investigate the localization and expression of CCDC69 in tissues and cells.
  • Western Blotting (WB): To detect the presence of CCDC69 protein in different samples .

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