CCDC25 Human

Coiled-Coil Domain Containing 25 Human Recombinant
Cat. No.
BT11638
Source
E.coli.
Synonyms
Coiled-coil domain-containing protein 25, CCDC25.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CCDC25 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 231 amino acids (1-208 a.a) and having a molecular mass of 26.9kDa.
CCDC25 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Coiled-Coil Domain Containing 25 (CCDC25) is a protein containing 208 amino acids. It is part of the CCDC25 family.
Description
Recombinant human CCDC25 is produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain that contains 231 amino acids (amino acids 1-208), with a molecular mass of 26.9 kDa. The protein has a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile and colorless solution.
Formulation
The CCDC25 protein solution has a concentration of 1 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
The product can be stored at 4°C for 2-4 weeks. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
Purity is greater than 90% as determined by SDS-PAGE.
Synonyms
Coiled-coil domain-containing protein 25, CCDC25.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVFYFTS SSVNSSAYTI YMGKDKYENE DLIKHGWPED IWFHVDKLSS AHVYLRLHKG ENIEDIPKEV LMDCAHLVKA NSIQGCKMNN VNVVYTPWSN LKKTADMDVG QIGFHRQKDV KIVTVEKKVN EILNRLEKTK VERFPDLAAE KECRDREERN EKKAQIQEMK KREKEEMKKK REMDELRSYS SLMKVENMSS NQDGNDSDEF M.

Product Science Overview

Gene Location and Structure

The CCDC25 gene is located on the short arm of human chromosome 8, specifically at 8p21.1 . The gene is transcribed into an mRNA product approximately 3,583 nucleotides in length . The protein itself is made up of a single polypeptide chain consisting of 208 amino acids .

Expression and Localization

CCDC25 is highly conserved among mammals and other eukaryotes, such as Arabidopsis thaliana . It is expressed ubiquitously in human tissues, with high expression levels in oocytes, Achilles tendon, duodenum, and other tissues . Despite its widespread expression, the function of CCDC25 is not well understood. It is predicted to localize to the nucleus and has been shown to be upregulated in Metaphase II oocytes .

Functional Insights

Although the precise function of CCDC25 remains unclear, it has been shown to interact with Smad2, a latent transcription factor involved in the TGF-β signaling pathway . Additionally, CCDC25 is a predicted substrate for several kinases, including Casein Kinase 1, Casein Kinase 2, cGMP-dependent protein kinase, and the Insulin Receptor . These interactions suggest that CCDC25 may play a role in cell signaling and regulation.

Clinical Relevance

CCDC25 has been implicated in various cellular processes, including the regulation of cell motility. It acts as a transmembrane receptor that senses neutrophil extracellular traps (NETs) and triggers the ILK-PARVB pathway to enhance cell motility . This function is particularly relevant in the context of cancer metastasis, where CCDC25 promotes the migration of tumor cells by sensing NETs .

Research and Future Directions

Given its potential roles in cell signaling and cancer metastasis, CCDC25 is a subject of ongoing research. Understanding its precise functions and interactions could provide valuable insights into cellular processes and disease mechanisms.

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