CCDC23 Human

Coiled-Coil Domain Containing 23 (CCDC23) is a protein that belongs to the family of coiled-coil domain-containing proteins. These proteins are characterized by the presence of coiled-coil motifs, which are structural motifs in proteins where 2-7 alpha-helices are coiled together like the strands of a rope. Coiled-coil domains are involved in a variety of biological processes, including structural support, signal transduction, and molecular recognition .

The coiled-coil domain is a versatile structural motif that can mediate protein-protein interactions. In the case of CCDC23, the coiled-coil domain allows it to interact with other proteins, potentially playing a role in cellular processes such as cytoskeletal organization and intracellular transport. The specific functions of CCDC23 are still under investigation, but its structural properties suggest it may be involved in forming protein complexes and facilitating cellular communication .

The recombinant form of CCDC23 can be produced using various expression systems, including bacterial, yeast, insect, and mammalian cells. The choice of expression system depends on the desired yield, post-translational modifications, and functional activity of the protein. Typically, the gene encoding CCDC23 is cloned into an expression vector, which is then introduced into the host cells. The recombinant protein is expressed, harvested, and purified using techniques such as affinity chromatography, ion exchange chromatography, and size exclusion chromatography .

The analysis of CCDC23 involves various biochemical and biophysical techniques to understand its structure, function, and interactions. Techniques such as circular dichroism (CD) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and X-ray crystallography are used to study the secondary and tertiary structures of the protein. Additionally, mass spectrometry and protein-protein interaction assays can provide insights into the molecular interactions and functional roles of CCDC23 .