CCDC101 Human
Description
CCDC101 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CCDC101 is a single polypeptide chain consisting of 313 amino acids, with a molecular mass of approximately 35.4 kDa. The recombinant form of this protein is typically produced in Escherichia coli (E. coli) and includes a 20 amino acid His-tag at the N-terminus to facilitate purification . The protein is purified using proprietary chromatographic techniques to achieve a purity greater than 90%, as determined by SDS-PAGE .
Coiled-coil domains are known for their versatility and are involved in a wide range of biological processes. CCDC101, like other coiled-coil domain-containing proteins, plays a role in various physiological and pathological processes. These roles include interactions with molecular components of signaling pathways, regulation of gene expression, and structural support .
The recombinant CCDC101 protein is formulated in a sterile, filtered, colorless solution containing 20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 1 mM DTT, and 10% glycerol. For short-term storage, the protein can be kept at 4°C if used within 2-4 weeks. For longer-term storage, it is recommended to store the protein at -20°C, with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s stability .
The study of coiled-coil domain-containing proteins like CCDC101 is crucial for understanding their diverse roles in cellular functions. Research on these proteins can provide insights into their involvement in signal transduction, gene regulation, and structural integrity. Additionally, the development of tools such as SamCC-Turbo has enabled high-throughput analysis of coiled-coil structures, facilitating the exploration of their properties and functions .
In summary, CCDC101 is a significant protein within the coiled-coil domain-containing family, with important roles in various biological processes. Its recombinant form, produced in E. coli, is widely used in research to further understand its functions and applications.
