CAV1 Antibody

Caveolin-1, Mouse Anti Human
Cat. No.
BT14002
Source
Synonyms
Caveolin-1, CAV1, CAV, CGL3, BSCL3, VIP21, MSTP085.
Appearance
Sterile Filtered clear solution.
Purity
Usage
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Description

Product Specs

Introduction
The CAV1 gene encodes a scaffolding protein crucial for forming caveolae plasma membranes present in most cell types. Caveolae formation and stability rely heavily on cholesterol, making their structure susceptible to changes in cholesterol levels or exposure to cholesterol-binding drugs. CAV1 demonstrates a strong and selective binding affinity for free cholesterol and artificial cholesterol-containing vesicles. This protein connects integrin subunits to the tyrosine kinase FYN, a crucial step in linking integrins to the Ras-ERK pathway, which stimulates cell cycle progression. Considered a potential tumor suppressor gene, CAV1 negatively regulates the Ras-p42/44 mitogen-activated kinase. Located adjacent to each other on chromosome 7, CAV1 and CAV2 express co-localizing proteins that assemble into a stable hetero-oligomeric complex cascade. Notably, mutations in the CAV1 gene are associated with Berardinelli-Seip congenital lipodystrophy.
Physical Appearance
Clear solution, sterile-filtered.
Formulation
1 mg/ml in PBS, pH 7.4, with 0.1% Sodium Azide.
Storage Procedures
Store at 4°C for up to 1 month. For extended storage, keep at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
The CAV1 antibody has undergone rigorous testing through ELISA and Western blot to ensure its specificity and reactivity. However, due to variations in applications, it is recommended to titrate the reagent for optimal results in each specific experiment. For Western blot analysis, a dilution range of 1:250 to 1:1000 is advised, with a starting dilution of 1:500 recommended.
Synonyms
Caveolin-1, CAV1, CAV, CGL3, BSCL3, VIP21, MSTP085.
Purification Method
CAV1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT4C1AT.
Immunogen
Anti-human CAV1 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CAV1 amino acids 1-104 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.

Product Science Overview

Introduction

Caveolin-1 (CAV1) is a scaffolding protein found within caveolar membranes. It plays a crucial role in various cellular processes, including signal transduction, lipid metabolism, and endocytosis. Caveolin-1 is ubiquitously expressed in many cell types and tissues, making it a significant protein for research in cell biology and pathology.

Structure and Function

Caveolin-1 is a 22-24 kDa protein that forms a hairpin-like structure within the plasma membrane. It is involved in the formation of caveolae, which are small invaginations on the cell surface. These structures are essential for various cellular functions, including:

  • Signal Transduction: Caveolin-1 interacts with G-protein alpha subunits, regulating their activity and thus influencing various signaling pathways.
  • Lipid Metabolism: It plays a role in lipid transport and homeostasis.
  • Endocytosis: Caveolin-1 is involved in the internalization of specific molecules and pathogens.
Role in Disease

Mutations in the CAV1 gene can lead to several diseases, including:

  • Congenital Generalized Lipodystrophy 3: A disorder characterized by the near-total absence of adipose tissue.
  • Pulmonary Hypertension: Increased blood pressure in the pulmonary arteries.
  • Partial Lipodystrophy/Congenital Cataracts and Neurodegeneration Syndrome: A condition involving abnormal fat distribution, cataracts, and neurological issues .
Mouse Anti-Human Caveolin-1 Antibodies

Mouse anti-human Caveolin-1 antibodies are widely used in research to study the expression and function of Caveolin-1 in human tissues. These antibodies are typically monoclonal, meaning they are derived from a single clone of cells and recognize a specific epitope on the Caveolin-1 protein. They are used in various applications, including:

  • Western Blotting: To detect Caveolin-1 protein levels in different samples.
  • Immunohistochemistry: To visualize Caveolin-1 expression in tissue sections.
  • Immunocytochemistry: To study Caveolin-1 in cultured cells.
  • Flow Cytometry: To analyze Caveolin-1 expression on the cell surface .
Research Applications

Caveolin-1 antibodies are valuable tools in research areas such as cancer, cardiovascular diseases, and metabolic disorders. For instance, Caveolin-1 has been studied as a biomarker for radiation resistance and tumor progression in lung cancer . Additionally, Caveolin-1 knockout mice have been used to investigate the protein’s role in pulmonary hypertension and obesity .

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