Cathepsin H Antibody

Cathepsin H, Mouse Anti Human
Cat. No.
BT14495
Source
Synonyms
Cathepsin H, CTSH, CPSB, ACC-4, ACC-5, MGC1519, minichain, DKFZp686B24257.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Cathepsin H, a lysosomal cysteine proteinase, plays a crucial role in the degradation of lysosomal proteins. It exists as a dimer composed of disulfide-linked heavy and light chains, both derived from a single protein precursor. As a member of the peptidase C1 protein family, Cathepsin H exhibits both aminopeptidase and endopeptidase activities. Notably, elevated expression of the Cathepsin H gene has been linked to the progression of prostate tumors.
Formulation
The antibody is supplied at a concentration of 1 mg/ml in a solution containing PBS (pH 7.4) and 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store the antibody at 4°C. For extended storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The Cathepsin H antibody has undergone rigorous testing by ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions may vary depending on the specific application. For Western blot analysis, a dilution range of 1:500 to 1:2,000 is recommended, with a starting dilution of 1:1,000.
Synonyms
Cathepsin H, CTSH, CPSB, ACC-4, ACC-5, MGC1519, minichain, DKFZp686B24257.
Purification Method
Cathepsin H antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P3G8AT.
Immunogen
Anti-human Cathepsin H mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Cathepsin H amino acids 23-335 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.

Product Science Overview

Introduction

Cathepsin H (CatH) is a lysosomal cysteine protease with unique aminopeptidase activity. It plays a crucial role in various physiological processes, including protein degradation, immune response, and cellular homeostasis . The mouse anti-human Cathepsin H antibody is a monoclonal antibody used in research to study the expression and function of CatH in human tissues and cells.

Structure and Function

Cathepsin H belongs to the family of cysteine cathepsins, which are proteases mainly located in the endolysosomal compartment of cells . It has both aminopeptidase and endopeptidase activities, allowing it to cleave peptide bonds at the amino terminus of proteins and within the protein chain . This dual activity makes CatH a versatile enzyme involved in various cellular processes.

Role in Immune Response

CatH is expressed in activated immune cells, including dendritic cells, macrophages, and microglia . It plays a critical role in the proteolytic maturation and stabilization of toll-like receptor 3 (TLR3), which is necessary for the production of interferon-beta (IFN-β) . IFN-β has neuroprotective effects and is involved in the immune response to viral infections .

Research Applications

The mouse anti-human Cathepsin H antibody is widely used in research to study the expression and function of CatH in various human tissues and cells. It is used in immunohistochemistry, immunofluorescence, and western blotting to detect CatH protein levels and localization . This antibody is also used to investigate the role of CatH in diseases such as cancer, neurodegenerative disorders, and immune-related conditions .

Clinical Implications

Dysregulated CatH activity has been implicated in various diseases, including cancer and neurodegenerative disorders . In cancer, CatH is involved in tumor progression and metastasis by promoting the degradation of extracellular matrix proteins and facilitating tumor cell invasion . In neurodegenerative disorders, CatH deficiency has been shown to impair TLR3-mediated activation of interferon regulatory factor 3 (IRF3), leading to reduced IFN-β production and increased neuronal cell death .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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