CARHSP1 Human
Calcium Regulated Heat Stable Protein 1 Human Recombinant
Cat. No.
BT29427
Source
Escherichia Coli.
Synonyms
Calcium-regulated heat stable protein 1, Calcium-regulated heat-stable protein of 24 kDa, CRHSP-24, CARHSP1, asCSDC1, MGC111446.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CRAHSP1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 167 amino acids (1-147 a.a.) and having a molecular mass of 18kDa. The CRAHSP1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
CRAHSP1, a serine phosphoprotein, serves as a physiological substrate for the Ca2+-calmodulin regulated protein phosphatase calcineurin (PP2B). This protein interacts with STYX/dead phosphate protein during spermatid development and shares homology with PIPPIN, a brain-specific mRNA-binding protein. CRAHSP1 is thought to play a role in calcium-mediated signal transduction through phosphorylation at serine residues.
Description
Recombinant human CRAHSP1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 167 amino acids, including a 20 amino acid His tag at the N-terminus (1-147 a.a. of the CRAHSP1 sequence), and has a molecular weight of 18kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The CRAHSP1 solution is provided at a concentration of 0.5 mg/ml in 20mM Tris-HCl buffer (pH 8.0), supplemented with 10% glycerol and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Calcium-regulated heat stable protein 1, Calcium-regulated heat-stable protein of 24 kDa, CRHSP-24, CARHSP1, asCSDC1, MGC111446.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSSEPPPPPQ PPTHQASVGL LDTPRSRERS PSPLRGNVVP SPLPTRRTRT FSATVRASQG PVYKGVCKCF CRSKGHGFIT PADGGPDIFL HISDVEGEYV PVEGDEVTYK MCSIPPKNEK LQAVEVVITH LAPGTKHETW SGHVISS.
Product Science Overview
Discovery and Function
CARHSP1 was originally identified as a substrate of the calcium/calmodulin-regulated protein phosphatase calcineurin . It is a paralog of the brain-specific cold shock protein PIPPin . CARHSP1 binds to and stabilizes tumor necrosis factor (TNF) mRNA within P-bodies and exosomes . This binding activity is crucial for the regulation of mRNA stability, which in turn affects the expression of various genes involved in inflammatory responses .
Biological Significance
CARHSP1 is involved in several important cellular processes:
- mRNA Stability: It binds to the 3’-UTR of mRNA, regulating its stability and thereby influencing gene expression .
- Response to Calcium: As its name suggests, CARHSP1 is regulated by calcium levels within the cell. This regulation is essential for its role in various signaling pathways .
- Cold Shock Response: Like other cold shock proteins, CARHSP1 is involved in the cellular response to stress, particularly cold shock .
Clinical Relevance
Mutations or dysregulation of CARHSP1 have been associated with several diseases:
- Chromosome 6Q24-Q25 Deletion Syndrome: This genetic disorder is linked to deletions in the region of chromosome 6 where the CARHSP1 gene is located .
- Ciliary Dyskinesia, Primary, 32: This condition is characterized by defects in the action of cilia lining the respiratory tract, leading to respiratory issues .
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