TNNT2 Human
Escherichia Coli.
CMH2, RCM3, TnTC, cTnT, CMPD2, MGC3889, TNNT2, Troponin T- cardiac muscle, Cardiac muscle troponin T, troponin T type 2 (cardiac).
Sterile filtered colorless solution.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Cardiac Muscle Troponin T Human Recombinant produced in E.Coli is a single, non- glycosylated polypeptide chain containing 297 amino acids and having a molecular mass of 35.8kDa.
TNNT2 is purified by proprietary chromatographic techniques.
Product Specs
CMH2, RCM3, TnTC, cTnT, CMPD2, MGC3889, TNNT2, Troponin T- cardiac muscle, Cardiac muscle troponin T, troponin T type 2 (cardiac).
Escherichia Coli.
SDIEEVVEEY EEEEQEEAAV EEEEDWREDE DEQEEAAEED AEAEAETEET RAEEDEEEEE AKEAEDGPME ESKPKPRSFM PNLVPPKIPD GERVDFDDIH RKRMEKDLNE LQALIEAHFE NRKKEEEELV SLKDRIERRR AERAEQQRIR NEREKERQNR LAEERARREE EENRRKAEDE ARKKKALSNM MHFGGYIQKQ AQTERKSGKR QTEREKKKKI LAERRKVLAI DHLNEDQLRE KAKELWQSIY NLEAEKFDLQ EKFKQQKYEI NVLRNRINDN QKVSKTRGKA KVTGRWK.
Product Science Overview
Cardiac Troponin T (cTnT) is a protein encoded by the TNNT2 gene in humans. It is a single, non-glycosylated polypeptide consisting of 305 amino acids with a molecular weight of approximately 36.4 kDa . The recombinant form of cTnT is produced in Escherichia coli and is often fused to a 20-amino acid His-tag at the N-terminus to facilitate purification .
The primary function of cTnT is to bind tropomyosin and anchor the troponin complex to the thin filament of the muscle fiber. This anchoring is essential for the regulation of muscle contraction in response to changes in intracellular calcium ion concentrations. When calcium ions bind to Troponin C (TnC), it induces conformational changes in the troponin complex, allowing the interaction between actin and myosin, leading to muscle contraction .
Mutations in the TNNT2 gene are associated with various cardiomyopathies, including familial hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) . These mutations can lead to altered troponin function, resulting in impaired muscle contraction and heart function. Measurement of cTnT levels in the blood is a widely used diagnostic marker for myocardial infarction (heart attack) and other cardiac injuries .
Recombinant human cTnT is used in various research and clinical applications, including:
- Diagnostic Assays: Measurement of cTnT levels in blood samples to diagnose myocardial infarction and other cardiac conditions .
- Research: Studying the molecular mechanisms of muscle contraction and the effects of mutations in the TNNT2 gene .
- Drug Development: Screening potential therapeutic compounds that target the troponin complex and modulate muscle contraction .
Recombinant human cTnT is typically supplied as a sterile-filtered, colorless solution in a buffer containing 20 mM Tris (pH 8.0) and 10% glycerol . It should be stored at 4°C if used within 2-4 weeks or at -20°C for longer periods. For long-term storage, the addition of 0.1% human serum albumin or bovine serum albumin is recommended to prevent degradation .
