Carboxypeptidase B Rat
Description
Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 31kDa is purified by standard chromatography techniques.
Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA.
Product Specs
Product Science Overview
Recombinant Carboxypeptidase B from rats is typically expressed in Escherichia coli (E. coli) or Pichia pastoris . The gene encoding the amino acid sequence of rat Carboxypeptidase B is chemically synthesized and transformed into these expression hosts. The recombinant enzyme produced in these systems exhibits properties identical to the native enzyme .
- Molecular Weight: Approximately 31-34.5 kDa .
- Optimal pH: Around 7.9 .
- Isoelectric Point (pI): Approximately 6 .
- Inhibition: Carboxypeptidase B is inhibited by basic amino acids like arginine, lysine, and ornithine. It is also inhibited by metal chelating agents such as EDTA and 1,10-phenanthroline .
The enzyme’s activity is measured by its ability to hydrolyze hippuryl-L-arginine. One unit of Carboxypeptidase B activity is defined as the amount of enzyme that hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65 . The biological activity of recombinant Carboxypeptidase B is approximately 170 units/mg protein .
Recombinant Carboxypeptidase B is used in various applications, including:
- Pharmaceutical Production: It is used as a critical raw material for the production of active pharmaceutical ingredients (APIs), such as insulin .
- Biochemical Research: The enzyme is employed in protein sequencing and peptide mapping due to its specificity for C-terminal basic amino acids .
- Industrial Processes: It is used in processes requiring high purity and consistent quality, such as the production of therapeutic proteins .
Recombinant Carboxypeptidase B is typically stored as a lyophilized powder or in a solution. The lyophilized form should be stored at 4°C and, upon reconstitution, should be stored at 4°C for up to two weeks or below -18°C for long-term storage . The enzyme is stable within the specified range for 12 months when stored at -15 to -25°C .
