Car b 1.0109
Greater than 95.0% as determined by SDS-PAGE.
Description
Recombinant Pollen allergen Car b 1 isoform produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 18kDa.
Car b 1.0109 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Pollen allergens are proteins found in pollen grains that can trigger allergic reactions in sensitive individuals. One such allergen is Car b 1, which is derived from the European hornbeam (Carpinus betulus). The recombinant form of this allergen, known as Pollen Allergen Car b 1 Isoform Recombinant, is produced using biotechnological methods to study its properties and potential applications.
The recombinant form of Car b 1 is produced in Escherichia coli (E. coli) expression systems. This involves inserting the gene encoding the Car b 1 protein into E. coli, which then produces the protein. The recombinant protein is typically tagged with a His tag at the N-terminus to facilitate purification .
The recombinant Car b 1 protein is a non-glycosylated polypeptide chain with a calculated molecular mass of approximately 18 kDa . It consists of 160 amino acids and belongs to the Bet v 1 family of allergens . The protein is expressed with high purity (>90%) and is suitable for various applications, including SDS-PAGE .
Car b 1 plays a role in the plant’s defense mechanisms and is involved in the abscisic acid-activated signaling pathway . In humans, it acts as an allergen, triggering immune responses in individuals with pollen allergies. The allergenic properties of Car b 1 are well-documented, and it is known to cause symptoms such as sneezing, runny nose, and itchy eyes in sensitive individuals .
Recombinant Car b 1 is used in various research applications, including:
- Allergy Diagnostics: It is used to develop diagnostic tests for pollen allergies.
- Immunotherapy: It is studied for its potential use in allergen-specific immunotherapy to desensitize allergic individuals.
- Structural Studies: The protein’s structure is analyzed to understand its allergenic properties and interactions with the immune system.
