CAPZA2 Human

Capping Protein (Actin Filament) Muscle Z-Line Alpha 2 Human Recombinant
Cat. No.
BT4261
Source
Escherichia Coli.
Synonyms
Capping Protein (Actin Filament) Muscle Z-Line, Alpha 2, F-Actin Capping Protein Alpha-2 Subunit, CapZ Alpha-2, CAPPA, CAPZ2, F-Actin-Capping Protein Subunit Alpha-2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CAPZA2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 309 amino acids (1-286 a.a) and having a molecular mass of 35.3kDa.
CAPZA2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Capping Protein (Actin Filament) Muscle Z-Line, Alpha 2, also known as CAPZA2, is part of the F-actin capping protein alpha subunit family. This protein is the alpha subunit of the barbed-end actin binding protein Cap Z. CAPZA2 plays a role in regulating the growth of actin filaments at the barbed end by capping them. Conditions associated with CAPZA2 include endocarditis and cervicitis.
Description
Recombinant human CAPZA2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 309 amino acids (specifically, amino acids 1-286) and has a molecular weight of 35.3 kDa. A 23 amino acid His-tag is fused to the N-terminus of CAPZA2. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The CAPZA2 protein solution has a concentration of 1 mg/ml. It is prepared in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4 M urea.
Stability
For short-term storage (2-4 weeks), the solution can be kept at 4°C. For extended storage, it should be frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
SDS-PAGE analysis indicates a purity greater than 85.0%.
Synonyms
Capping Protein (Actin Filament) Muscle Z-Line, Alpha 2, F-Actin Capping Protein Alpha-2 Subunit, CapZ Alpha-2, CAPPA, CAPZ2, F-Actin-Capping Protein Subunit Alpha-2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADLEEQ LSDEEKVRIA AKFIIHAPPG EFNEVFNDVR LLLNNDNLLR EGAAHAFAQY NLDQFTPVKI EGYEDQVLIT EHGDLGNGKF LDPKNRICFK FDHLRKEATD PRPCEVENAV ESWRTSVETA LRAYVKEHYP NGVCTVYGKK IDGQQTIIAC IESHQFQAKN FWNGRWRSEW KFTITPSTTQ VVGILKIQVH YYEDGNVQLV SHKDIQDSLT VSNEVQTAKE FIKIVEAAEN EYQTAISENY QTMSDTTFKA LRRQLPVTRT KIDWNKILSY KIGKEMQNA.

Product Science Overview

Introduction

Capping Protein (Actin Filament) Muscle Z-Line Alpha 2, also known as CAPZA2, is a crucial protein involved in the regulation of actin filament dynamics. This protein is a part of the F-actin capping protein alpha subunit family and plays a significant role in muscle function by capping the barbed ends of actin filaments, thereby regulating their growth and stability .

Gene and Protein Structure

The CAPZA2 gene is located on human chromosome 7, specifically at position q31.2-q31.3 . The gene encodes a protein that is approximately 33.0 kDa in size and composed of 286 amino acids . The primary sequence of CapZ-alpha2 contains three C-terminal, regularly spaced leucines at positions 258, 262, and 266, which are critical for actin binding . These residues are conserved within the CapZ-beta isoform, highlighting the importance of these sequences in the protein’s function .

Function

CapZ-alpha2 binds to the barbed ends of actin filaments in a calcium-independent manner, preventing the addition or loss of actin monomers at these ends . This capping action is essential for the regulation of actin filament dynamics, which is crucial for various cellular processes, including cell motility, division, and muscle contraction . Unlike other capping proteins such as gelsolin and severin, CapZ-alpha2 does not sever actin filaments .

Role in Muscle Function

CapZ-alpha2 is predominantly expressed in cardiac and skeletal muscle tissues, where it caps sarcomeric actin at Z-discs . This capping action is vital for the organization and stability of myofilaments during myofibrillogenesis, the process by which muscle fibers are formed . CapZ-alpha2 is present at Z-discs in muscle cells before the striation of actin filaments, suggesting that it plays a role in directing the polarity and organization of sarcomeric actin during I-band formation .

Clinical Significance

Mutations or dysregulation of the CAPZA2 gene have been associated with various diseases, including nonobstructive coronary artery disease and autosomal recessive deafness . Understanding the function and regulation of CapZ-alpha2 is crucial for developing therapeutic strategies for these conditions.

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