CAPS Human

Calcyphosine Human Recombinant
Cat. No.
BT2132
Source
Escherichia Coli.
Synonyms
Calcyphosin, Calcyphosine, CAPS, CAPS1, MGC126562.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CAPS Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 209 amino acids (1-189 a.a.) and having a molecular mass of 23.1kDa. The CAPS is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Calcyphosine (CAPS) is a calcium-binding protein that plays a role in regulating ion transport. It contains four EF-hand domains and is found in various tissues, including epithelial cells and some central nervous system cells. CAPS was first discovered in thyroid follicular cells, where it is synthesized and phosphorylated in response to thyrotropin and cAMP agonists.
Description
Recombinant human CAPS protein is produced in E. coli and is fused to a 20 amino acid His tag at the N-terminus. This results in a single, non-glycosylated polypeptide chain containing 209 amino acids (residues 1-189 of the CAPS protein) with a molecular weight of 23.1 kDa. The protein is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CAPS protein is supplied as a 1 mg/ml solution in 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT, and 100mM NaCl.
Stability
For short-term storage (up to 4 weeks), the CAPS protein can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the CAPS protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Calcyphosin, Calcyphosine, CAPS, CAPS1, MGC126562.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDAVDATMEK LRAQCLSRGA SGIQGLARFF RQLDRDGSRS LDADEFRQGL AKLGLVLDQA EAEGVCRKWD RNGSGTLDLE EFLRALRPPM SQAREAVIAA AFAKLDRSGD GVVTVDDLRG VYSGRAHPKV RSGEWTEDEV LRRFLDNFDS SEKDGQVTLA EFQDYYSGVS ASMNTDEEFV AMMTSAWQL.

Product Science Overview

Gene and Protein Structure

The CAPS gene encodes the calcyphosine protein, which contains four EF-hand domains, typical of calcium-binding proteins . The gene is located on chromosome 19 in humans and has several aliases, including CAPS1 and MGC126562 .

Function and Mechanism

Calcyphosine is involved in the regulation of ion transport. In thyroid follicular cells, it is synthesized and phosphorylated in response to stimulation by thyrotropin and cAMP agonists . This protein may play a role in various cellular signaling pathways, although its exact mechanisms are still under investigation.

Recombinant Calcyphosine

Recombinant human calcyphosine is produced using Escherichia coli (E. coli) expression systems. The recombinant protein typically includes an N-terminal His-tag to facilitate purification . It is used in various research applications, including studies on calcium-binding proteins and their roles in cellular processes.

Clinical and Research Applications

Calcyphosine has been associated with several diseases, including bone resorption disease and giant papillary conjunctivitis . Its role in these conditions is still being explored, but its involvement in calcium signaling makes it a protein of interest in both clinical and research settings.

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Source
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THE BIOTEK
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