CANX Antibody

Calnexin is a calcium-binding chaperone protein that resides in the endoplasmic reticulum (ER) of eukaryotic cells. It plays a crucial role in the quality control of newly synthesized glycoproteins, ensuring proper folding and preventing misfolded proteins from being transported to their final destinations .

Calnexin is a type I integral membrane protein with a large luminal domain, a single transmembrane helix, and a short cytoplasmic tail. The luminal domain contains the carbohydrate-binding site, which interacts with the glycan moieties of nascent glycoproteins. This interaction is essential for the retention of these glycoproteins in the ER until they achieve their correct conformation .

Calnexin, along with its homolog calreticulin, forms part of the calnexin/calreticulin cycle, which is a key component of the ER quality control system. This cycle ensures that only properly folded glycoproteins proceed to the Golgi apparatus, while misfolded proteins are targeted for degradation .

The Mouse Anti-Human Calnexin antibody is a monoclonal antibody that specifically targets the calnexin protein in human cells. Monoclonal antibodies are produced by identical immune cells that are clones of a unique parent cell, ensuring specificity and consistency in their binding to the target antigen .

This antibody is widely used in various scientific applications, including:

• Western Blotting (WB): To detect calnexin protein levels in different tissue samples.
• Immunohistochemistry (IHC): To visualize the localization of calnexin in tissue sections.
• Immunocytochemistry (ICC): To study the distribution of calnexin within cultured cells.
• Immunoprecipitation (IP): To isolate calnexin from cell lysates for further analysis .

The Mouse Anti-Human Calnexin antibody is an invaluable tool in biomedical research. It helps scientists understand the role of calnexin in various cellular processes, including protein folding, ER stress response, and the pathogenesis of diseases related to protein misfolding, such as neurodegenerative disorders and certain types of cancer .

By using this antibody, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets. Additionally, the antibody’s specificity and reliability make it a preferred choice for experiments requiring precise detection of calnexin .