Calmodulin Antibody
Description
Product Specs
Calmodulin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Product Science Overview
Calmodulin is a small, highly conserved calcium-binding protein found in all eukaryotic cells. It plays a crucial role in various cellular processes by mediating the control of numerous enzymes, ion channels, aquaporins, and other proteins through its interaction with calcium ions (Ca2+). The protein can bind up to four calcium ions, which induces conformational changes that enhance its affinity for target proteins.
Calmodulin is a 17 kDa protein composed of 148 amino acids. It has two globular domains, each containing two EF-hand motifs that bind calcium ions. Upon binding calcium, calmodulin undergoes a conformational change that allows it to interact with and regulate a variety of target proteins. These interactions are essential for processes such as energy metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular signaling.
Calmodulin is involved in several critical cellular processes, including:
- Regulation of Enzymes: Calmodulin activates various protein kinases and phosphatases, which are essential for signal transduction pathways.
- Ion Channel Regulation: It modulates the activity of ion channels, influencing cellular excitability and signaling.
- Cytoskeletal Dynamics: Calmodulin interacts with cytoskeletal proteins, playing a role in cell shape and motility.
- Exocytosis: It is involved in the release of neurotransmitters and hormones through its interaction with proteins involved in vesicle fusion.
Mouse anti-human calmodulin antibodies are monoclonal antibodies developed in mice that specifically target human calmodulin. These antibodies are widely used in various scientific applications, including Western blotting, immunohistochemistry, immunocytochemistry, ELISA, and flow cytometry. They are valuable tools for studying calmodulin’s role in different biological processes and for detecting its presence in various samples.
- Western Blotting: Used to detect calmodulin in protein samples separated by gel electrophoresis.
- Immunohistochemistry: Allows for the visualization of calmodulin in tissue sections.
- Immunocytochemistry: Used to detect calmodulin in cultured cells.
- ELISA: Enables the quantification of calmodulin in various samples.
- Flow Cytometry: Allows for the analysis of calmodulin expression in cell populations.
