CA13 Human

Carbonic Anhydrase XIII Human Recombinant
Cat. No.
BT29120
Source
Escherichia Coli.
Synonyms
Carbonic Anhydrase XIII, Carbonate Dehydratase XIII, EC 4.2.1.1, CA-XIII, Carbonic, Anhydrase 13, CAXIII.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CA13 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (1-262a.a) and having a molecular mass of 31.8kDa.
CA13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Carbonic Anhydrase XIII, also known as CA13, is part of the alpha-carbonic anhydrase family. This family catalyzes the rapid conversion of carbon dioxide and water into bicarbonate and protons. This reaction is reversible and happens much slower without a catalyst. Almost all carbonic anhydrases contain a zinc ion in their active site, classifying them as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ, and ε), with no significant amino acid sequence resemblance, suggesting convergent evolution. The α-CAs are present in humans.
Description
Recombinant CA13 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain. It contains 285 amino acids (1-262a.a), with a molecular mass of 31.8 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution, sterilized by filtration.
Formulation
The CA13 protein solution (0.5 mg/ml) is supplied in a buffer containing Phosphate buffered saline (pH 7.4), 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Biological Activity
The specific activity, defined as the amount of enzyme that hydrolyzes 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37°C, is greater than 2,500 pmol/min/ug.
Synonyms
Carbonic Anhydrase XIII, Carbonate Dehydratase XIII, EC 4.2.1.1, CA-XIII, Carbonic, Anhydrase 13, CAXIII.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSP MGSMSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH.

Product Science Overview

Introduction

Carbonic Anhydrase XIII (CA XIII) is a member of the alpha-carbonic anhydrase family, which consists of zinc-containing metalloenzymes. These enzymes catalyze the reversible hydration of carbon dioxide, a crucial reaction for maintaining pH homeostasis in various tissues and organs .

Structure and Function

CA XIII is a cytosolic isozyme, meaning it is located within the cell’s cytoplasm. It is involved in the rapid interconversion of carbon dioxide and water to bicarbonate and protons. This reaction is essential for several physiological processes, including respiration, renal acidification, and the formation of cerebrospinal fluid and gastric acid .

Expression and Localization

CA XIII is expressed in a variety of normal tissues, including the brain, liver, and kidneys. Its expression is regulated by various factors, and it plays a role in maintaining the acid-base balance in these tissues . In cancer research, CA XIII has been observed to have down-regulated expression in tumor cells compared to normal tissues, suggesting a potential role in tumor suppression .

Recombinant Human CA XIII

Recombinant human CA XIII is produced using E. coli expression systems. The recombinant protein is typically purified to a high degree of purity (>95%) and is used in various research applications. It is often tagged with a histidine tag to facilitate purification and detection .

Applications

Recombinant CA XIII is used in biochemical studies to understand its enzymatic activity and role in physiological processes. It is also used in drug discovery and development, particularly in the context of diseases where pH regulation is disrupted .

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