CA11 Human

Carbonic Anhydrase XI Human Recombinant
Cat. No.
BT29029
Source
Escherichia Coli.
Synonyms
Carbonic Anhydrase XI, Carbonic Anhydrase-Related Protein 2, Carbonic Anhydrase-Related Protein 11, CARP-2, CA-RP II, CARP XI, CARPX1, CA-XI.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CA11 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 326 amino acids (24-328) and having a molecular mass of 36.3kDa.
CA11 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Carbonic anhydrases (CAs) constitute a large family of zinc-containing enzymes that facilitate the reversible hydration of carbon dioxide. These enzymes play crucial roles in various physiological processes, including respiration, maintaining acid-base equilibrium, calcification, bone resorption, and the production of essential fluids such as aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CAs exhibit significant diversity in their tissue distribution and subcellular localization. Carbonic Anhydrase XI (CA11), while potentially a secreted protein, is unlikely to possess carbonic anhydrase activity due to substantial alterations in active site residues that are otherwise conserved in catalytically active CA isozymes. CA11 shares characteristics with two other acatalytic CA isoforms, CA VIII and CA X. Notably expressed in the brain, CA11 is suggested to have a broad functional role within the central nervous system.
Description
Recombinantly produced in E. coli, CA11 Human is a single, non-glycosylated polypeptide chain comprising 326 amino acids (residues 24-328), resulting in a molecular weight of 36.3 kDa. This protein is engineered with a 21 amino acid His-tag fused at its N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The CA11 solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, consider adding a carrier protein such as HSA or BSA (0.1%). It is important to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the product is determined to be greater than 85% as assessed by SDS-PAGE analysis.
Synonyms
Carbonic Anhydrase XI, Carbonic Anhydrase-Related Protein 2, Carbonic Anhydrase-Related Protein 11, CARP-2, CA-RP II, CARP XI, CARPX1, CA-XI.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MHIGPAPDPE DWWSYKDNLQ GNFVPGPPFW GLVNAAWSLC AVGKRQSPVD VELKRVLYDP FLPPLRLSTG GEKLRGTLYN TGRHVSFLPA PRPVVNVSGG PLLYSHRLSE LRLLFGARDG AGSEHQINHQ GFSAEVQLIH FNQELYGNFS AASRGPNGLA ILSLFVNVAS TSNPFLSRLL NRDTITRISY KNDAYFLQDL SLELLFPESF GFITYQGSLS TPPCSETVTW ILIDRALNIT SLQMHSLRLL SQNPPSQIFQ SLSGNSRPLQ PLAHRALRGN RDPRHPERRC RGPNYRLHVD GVPHGR

Product Science Overview

Carbonic Anhydrase XI (CA XI)

Carbonic Anhydrase XI (CA XI), also known as CARP XI, is one of the acatalytic members of the carbonic anhydrase family. It is encoded by the CA11 gene, located on chromosome 19q13.33 . Unlike other carbonic anhydrases, CA XI lacks enzymatic activity due to the absence of one or more zinc-binding histidine residues .

Expression and Function

CA XI is predominantly expressed in the brain, with strong expression in the cerebellum, cerebral cortex, occipital lobe, frontal lobe, temporal lobe, and putamen . It is also expressed in the spinal cord and thyroid, albeit at lower levels . Despite its lack of enzymatic activity, CA XI is believed to play a crucial role in the central nervous system, possibly in signal transduction or cellular communication .

Recombinant CA XI

Recombinant CA XI is produced in E. coli as a single, non-glycosylated polypeptide chain containing 326 amino acids and having a molecular mass of 36.3 kDa . It is fused to a 21 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques .

Research and Applications

Research on CA XI has provided insights into its evolutionary conservation and potential functional roles. Studies have shown that CA XI has evolved very slowly in mammals, suggesting an important function despite its lack of enzymatic activity . The recombinant form of CA XI is used in various research applications to study its structure, function, and potential therapeutic uses.

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