CA10 Human

Carbonic anhydrases (CAs) are a family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide, producing bicarbonate and a proton. This reaction is fundamental to many physiological processes, including respiration, renal tubular acidification, and bone resorption . Among the various isoforms of carbonic anhydrase, Carbonic Anhydrase X (CA10) is a unique member with distinct properties and functions.

Carbonic Anhydrase X (CA10) is a protein encoded by the CA10 gene in humans. Unlike some other carbonic anhydrases, CA10 lacks catalytic activity for the hydration of carbon dioxide. Instead, it is believed to play a role in the central nervous system, particularly in brain development and function .

The recombinant form of Carbonic Anhydrase X (Human Recombinant) is produced using advanced biotechnological methods. Typically, it is expressed in a mouse myeloma cell line (NS0) and purified to high levels of purity, often exceeding 95% as determined by SDS-PAGE . The recombinant protein is usually tagged with a C-terminal 10-His tag to facilitate purification and detection.

Recombinant Carbonic Anhydrase X is used in various research applications, particularly in studies related to the nervous system. Its role in brain development makes it a valuable tool for understanding neurological disorders and potential therapeutic targets. Additionally, it is used in structural biology studies to elucidate the protein’s structure and interactions with other molecules .

The recombinant protein is typically lyophilized from a solution containing Tris and NaCl and should be reconstituted in a buffer solution before use. It is recommended to store the protein at -20 to -70°C to maintain its stability and avoid repeated freeze-thaw cycles .