C7 Human
Human Plasma.
Complement component C7, C7.
Sterile filtered solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
Human Complement C7 produced in Human plasma having a molecular mass of 92.4kDa.
Product Specs
Complement component C7 is crucial for the creation of the membrane attack complex (MAC). Its activation occurs upon binding to C5b-C6 complexes already present on the cell membrane. Each complement activation pathway generates enzyme complexes that attach to the target surface. These enzymes cleave C5, a key step in MAC formation. This cleavage releases C5a and activates C5b. While C5b is short-lived, it quickly binds to C6. This C5b-C6 complex can then bind a single C7 molecule, leading to the exposure of a membrane-binding site. This allows the C5b-6-7 complex to insert itself into the target cell's membrane, a critical step in MAC assembly.
This product consists of human complement component C7. It is derived from human plasma and possesses a molecular weight of 92.4 kDa.
This product is a sterile-filtered solution.
This C7 protein solution is buffered at a pH of 7.3 using 10mM Sodium phosphate and 145mM NaCl.
For optimal stability, store Human C7 at 4°C for usage within 2-4 weeks. For extended storage, freeze at temperatures below -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
The purity of this product exceeds 90.0% as assessed by SDS-PAGE.
Plasma used in the production of this product has undergone rigorous testing and is confirmed negative for antibodies against HIV-1, HIV-2, HCV, and HBSAG.
Complement component C7, C7.
Human Plasma.
Product Science Overview
C7 is an essential component of the membrane attack complex (MAC), which also includes complement components C5b, C6, C8, and C9 . The primary function of C7 is to bind to the C5b-C6 complex, forming a stable complex that facilitates the subsequent binding of C8 and C9 . This binding exposes a hydrophobic site on C7, allowing it to insert into the phospholipid bilayer of the pathogen . The resulting MAC creates pores in the pathogen’s membrane, leading to cell lysis and death .
C7 is expressed in various tissues, including the right ovary, superficial temporal artery, pericardium, right adrenal cortex, gastric mucosa, left adrenal gland, left adrenal cortex, gallbladder, right auricle, and right coronary artery . It is also found in the zone of skin, spermatid, esophagus, lung, testicle, spermatocyte, white adipose tissue, skeletal muscle tissue, adrenal gland, and quadriceps femoris muscle .
The primary biological functions of C7 include:
- Complement activation: C7 is involved in the activation of the complement system, particularly in the formation of the MAC .
- Cytolysis: By forming pores in the membranes of pathogens, C7 contributes to their destruction .
- Regulation of complement activation: C7 plays a role in regulating the complement system to prevent excessive inflammation and tissue damage .
