C4BPB Human

Complement Component 4 Binding Protein, Beta Human Recombinant

Cat. No.

BT16696

Source

E.coli.

Synonyms

C4b-binding protein beta chain, C4BPB, C4BP.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

C4BPB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 260 amino acids (18-252 a.a) and having a molecular mass of 29kDa.
C4BPB is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

C4BPB (Complement Component 4 Binding Protein, Beta) is a protein that plays a crucial role in regulating the complement system, a part of the immune system. It belongs to a family of proteins characterized by repeating structural units. C4BPB primarily functions by inhibiting the complement cascade, preventing excessive activation that can damage the body's own tissues. It achieves this by acting as a cofactor for C3bINA, an enzyme that inactivates complement components C3b and C4b. Moreover, C4BPB facilitates the breakdown of the C4bC2a complex, further controlling complement activation. Additionally, C4BPB interacts with Protein S, a protein involved in blood clotting regulation, and serum amyloid P component, highlighting its diverse roles in immune and coagulation systems.

Description

This product consists of a recombinant human C4BPB protein produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain, meaning it lacks attached sugar molecules. The protein contains 260 amino acids, with a molecular weight of 29kDa. For purification and detection purposes, a 25 amino acid His-tag is attached to the N-terminus of the protein. The purification process utilizes proprietary chromatographic techniques to ensure high purity.

Physical Appearance

Clear, colorless solution that has been sterilized by filtration.

Formulation

The C4BPB protein is provided in a solution with a concentration of 1mg/ml. The solution contains 20mM Tris-HCl buffer at pH 8.0, 10% glycerol, and 0.15M NaCl. These components help maintain the protein's stability and activity.

Stability

For short-term storage (up to 4 weeks), the product can be stored at 4°C. For longer-term storage, it is recommended to store the product frozen at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA at a concentration of 0.1%. It's important to avoid repeated freezing and thawing of the product to prevent protein degradation.

Purity

The purity of the C4BPB protein is greater than 90%, as determined by SDS-PAGE analysis, a widely used technique for assessing protein purity.

Synonyms

C4b-binding protein beta chain, C4BPB, C4BP.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSDAEH CPELPPVDNS IFVAKEVEGQ ILGTYVCIKG YHLVGKKTLF CNASKEWDNT TTECRLGHCP DPVLVNGEFS SSGPVNVSDK ITFMCNDHYI LKGSNRSQCL EDHTWAPPFP ICKSRDCDPP GNPVHGYFEG NNFTLGSTIS YYCEDRYYLV GVQEQQCVDG EWSSALPVCK LIQEAPKPEC EKALLAFQES KNLCEAMENF MQQLKESGMT MEELKYSLEL KKAELKAKLL.

Product Science Overview

Structure and Function

C4BPB is a component of the C4b-binding protein (C4BP), which is a large glycoprotein composed of seven identical alpha-chains and one unique beta-chain. The beta-chain of C4BP binds to protein S, a vitamin K-dependent protein that serves as a cofactor for activated protein C, which is involved in the regulation of blood coagulation. This interaction highlights the dual role of C4BP in both the complement system and the coagulation system.

The primary function of C4BP is to regulate the classical pathway of complement activation. It acts as a cofactor for the enzyme C3b/C4b inactivator (C3bINA), which hydrolyzes the complement fragment C4b. Additionally, C4BP accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. This regulatory role is crucial in preventing the over-activation of the complement system, which can lead to tissue damage and inflammation.

Clinical Significance

Mutations or dysregulation of the C4BPB gene can be associated with various diseases. For instance, Hemolytic Uremic Syndrome (HUS) and Atypical Hemolytic-Uremic Syndrome (aHUS) are linked to abnormalities in the complement system, including C4BPB. These conditions are characterized by the destruction of red blood cells, kidney failure, and low platelet count.

Moreover, C4BPB has been studied for its potential role as a biomarker. For example, the alpha-chain of C4BP (C4BPα) has been suggested as a biomarker to predict clopidogrel resistance in patients with coronary artery disease. Elevated levels of C4BPα in plasma may indicate a higher sensitivity to clopidogrel, a medication used to prevent blood clots.

Recombinant C4BPB

Recombinant human C4BPB is produced using recombinant DNA technology, which involves inserting the gene encoding C4BPB into a suitable expression system, such as Escherichia coli (E. coli). The recombinant protein is then purified using conventional chromatography techniques. This recombinant form is used in various research applications to study the function and regulation of the complement system and its role in disease.

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C4BPB Human

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