C1q Human, Liquid
Sterile Filtered solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
Human Complement Component C1q produced in Human plasma having a molecular mass of 410 kDa.
Product Specs
Human Complement Component C1q, derived from human plasma, has a molecular weight of 410 kDa.
Sterile Filtered solution.
The solution contains 10 mM HEPES and 300mM NaCl, with a pH of 7.2.
Human C1q can be stored at 4°C for 2-4 weeks if the entire vial will be used. For longer storage, freeze below -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
The purity is greater than 95.0% as determined by SDS-PAGE analysis.
Plasma from each donor undergoes testing to ensure it's negative for antibodies against HIV-1, HIV-2, HCV, and HBSAG.
Product Science Overview
Complement Component C1q is a glycoprotein composed of 18 polypeptide chains. These chains are organized into three nonidentical subunits, known as A, B, and C, with molecular weights of 29 kDa, 26 kDa, and 19 kDa, respectively . The structure of C1q is quite unique, resembling a bouquet of flowers, with six A-chains, six B-chains, and six C-chains forming a complex structure . Each chain contains a collagen-like region near the N-terminus and a C-terminal globular region .
C1q is the first component of the classical pathway of the complement system. It is present in normal human serum at a concentration of approximately 70 µg/ml . C1q, along with C1r and C1s, forms the C1 complex. This complex is responsible for the activation of the classical complement pathway, which is triggered by the binding of C1q to immune complexes containing immunoglobulin G (IgG) or immunoglobulin M (IgM) .
Upon binding to these immune complexes, C1q undergoes a conformational change that activates C1r and C1s, converting them into proteolytic enzymes. These enzymes then cleave other complement proteins, leading to a cascade of reactions that result in the opsonization of pathogens, recruitment of inflammatory cells, and lysis of target cells .
C1q plays a vital role in the clearance of apoptotic cells and immune complexes, preventing the accumulation of cellular debris and autoantigens that could trigger autoimmune responses . Deficiencies in C1q are associated with a higher risk of developing autoimmune diseases such as systemic lupus erythematosus . Additionally, C1q has been found to bind to apoptotic bodies of human keratinocytes, vascular endothelial cells, and lymphocytes, facilitating their clearance .
Complement Component C1q is widely used in research to study the mechanisms of the complement system and its role in immune responses. It is also used in diagnostic assays to evaluate the functionality of the classical complement pathway. In therapeutic contexts, understanding the role of C1q can aid in the development of treatments for autoimmune diseases and conditions involving immune complex-mediated pathology .
