Core 1 Beta3-Gal-T1, also known as C1GALT1, is an enzyme that plays a crucial role in the biosynthesis of O-glycans. It is responsible for creating the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by transferring galactose (Gal) from UDP-Gal to N-acetylgalactosamine (GalNAc)-alpha-1-R . This structure is a precursor for many extended mucin-type O-glycans found on cell surfaces and secreted glycoproteins .
The human recombinant form of C1GALT1 is produced in Escherichia coli (E. coli). It is a single, non-glycosylated polypeptide chain containing 357 amino acids (30-363 a.a) and has a molecular mass of approximately 41.4 kDa . The recombinant protein is fused to a 23 amino acid His-tag at the N-terminus, which aids in its purification through chromatographic techniques .
The recombinant form of C1GALT1 is used extensively in research to study its role in glycosylation and its impact on various biological processes. It is also utilized in the development of therapeutic interventions targeting glycosylation pathways.
The C1GALT1 protein solution is typically formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT . For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to prevent multiple freeze-thaw cycles .