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The C17ORF103 gene is part of the open reading frame (ORF) family, which includes genes that encode proteins with diverse functions. The human recombinant C17ORF103 protein is a single, non-glycosylated polypeptide chain consisting of 136 amino acids. The protein has a molecular mass of approximately 15.4 kDa . Additionally, the recombinant protein is often fused to a 23 amino acid His-tag at the N-terminus to facilitate purification .
The production of recombinant C17ORF103 involves cloning the gene into an expression vector, which is then introduced into a host organism, such as E. coli. The host organism expresses the protein, which can then be harvested and purified. The purification process typically involves chromatographic techniques, which take advantage of the His-tag to isolate the protein from other cellular components .
While the specific functions of C17ORF103 are still being studied, proteins encoded by open reading frames on chromosome 17 are known to play roles in various cellular processes, including signal transduction, cell cycle regulation, and apoptosis. The study of recombinant forms of these proteins helps researchers understand their functions and potential implications in health and disease.
Recombinant C17ORF103 is used in various research applications, including: