C17ORF103 Human

Chromosome 17 Open Reading Frame 103 Human Recombinant
Cat. No.
BT9383
Source
Escherichia Coli.
Synonyms
Chromosome 17 Open Reading Frame 103, Transcript Expressed During Hematopoiesis 2, Gene Trap Locus F3b, Gtlf3b, Protein GTLF3B.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

C17ORF103 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 136 amino acids (1-113) and having a molecular mass of 15.4kDa.
C17ORF103 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
C17orf103, a member of the GTLF3B family, is encoded by a gene situated on human chromosome 17p11.2. This chromosomal region is also linked to two significant tumor suppressor genes, BRCA1 and p53. The tumor suppressor p53 plays a crucial role in maintaining cellular genetic integrity by regulating cell fate decisions, such as DNA repair versus cell death. Additionally, C17orf103 is associated with neurofibromatosis, a disorder characterized by neural and epidermal lesions and abnormal Schwann cell growth.
Description
Recombinant human C17ORF103, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 136 amino acids (residues 1-113). It has a molecular weight of 15.4 kDa. For purification purposes, C17ORF103 is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The C17ORF103 solution is supplied in 20mM Tris-HCl buffer with a pH of 8.0, 0.1M NaCl, 1mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Multiple freeze-thaw cycles should be avoided.
Purity
Purity is determined to be greater than 95% as assessed by SDS-PAGE.
Synonyms
Chromosome 17 Open Reading Frame 103, Transcript Expressed During Hematopoiesis 2, Gene Trap Locus F3b, Gtlf3b, Protein GTLF3B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAHSAAA VPLGALEQGC PIRVEHDRRR RQFTVRLNGC HDRAVLLYEY VGKRIVDLQH TEVPDAYRGR GIAKHLAKAA LDFVVEEDLK AHLTCWYIQK YVKENPLPQY LERLQP

Product Science Overview

Gene and Protein Structure

The C17ORF103 gene is part of the open reading frame (ORF) family, which includes genes that encode proteins with diverse functions. The human recombinant C17ORF103 protein is a single, non-glycosylated polypeptide chain consisting of 136 amino acids. The protein has a molecular mass of approximately 15.4 kDa . Additionally, the recombinant protein is often fused to a 23 amino acid His-tag at the N-terminus to facilitate purification .

Production and Purification

The production of recombinant C17ORF103 involves cloning the gene into an expression vector, which is then introduced into a host organism, such as E. coli. The host organism expresses the protein, which can then be harvested and purified. The purification process typically involves chromatographic techniques, which take advantage of the His-tag to isolate the protein from other cellular components .

Functional Significance

While the specific functions of C17ORF103 are still being studied, proteins encoded by open reading frames on chromosome 17 are known to play roles in various cellular processes, including signal transduction, cell cycle regulation, and apoptosis. The study of recombinant forms of these proteins helps researchers understand their functions and potential implications in health and disease.

Applications in Research

Recombinant C17ORF103 is used in various research applications, including:

  • Protein-Protein Interaction Studies: Understanding how C17ORF103 interacts with other proteins can provide insights into its role in cellular processes.
  • Functional Assays: Researchers can study the effects of C17ORF103 on cell behavior, such as proliferation and apoptosis.
  • Structural Studies: Determining the three-dimensional structure of C17ORF103 can help elucidate its function and potential interactions with other molecules.

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