Introduction
C11ORF31, also known as Chromosome 11 Open Reading Frame 31, is a selenoprotein. Selenoproteins are characterized by the presence of a selenocysteine (Sec) amino acid at their active site. Unlike standard amino acids, Sec is encoded by the UGA codon, typically recognized as a stop signal for translation. To ensure the incorporation of Sec, selenoprotein genes possess a unique stem-loop structure in their 3' UTR called the selenocysteine insertion sequence (SECIS). SECIS acts as a signal for the cellular machinery to interpret UGA as a Sec codon instead of a termination signal. While the precise function of the C11ORF31 gene remains to be fully elucidated, selenoproteins are broadly implicated in various biological processes, particularly those involving redox reactions. It is suggested that C11ORF31 plays a role in redox regulation and might contribute to the beneficial health effects associated with dietary selenium intake.
Description
Recombinant human C11ORF31, expressed in E. coli, is a single polypeptide chain that lacks glycosylation. It comprises 145 amino acids, with residues 1-122 representing the C11ORF31 sequence, and has a molecular weight of 15.8 kDa. The recombinant protein features a 23 amino acid His-tag fused at its N-terminus to facilitate purification. The purification process employs proprietary chromatographic techniques to ensure high purity.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The provided C11ORF31 solution has a concentration of 0.5 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the C11ORF31 solution should be kept refrigerated at 4°C. For long-term storage, it is recommended to store the solution at -20°C in a frozen state. To enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA to a final concentration of 0.1%. It is crucial to avoid repeated cycles of freezing and thawing to prevent protein degradation.
Purity
The purity of the C11ORF31 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Chromosome 11 Open Reading Frame 31, Selenoprotein H, SELH, C17orf10, C11orf31.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPRGRK RKAEAAVVAV AEKREKLANG GEGMEEATVV IEHCTSCRVY GRNAAALSQA LRLEAPELPV KVNPTKPRRG SFEVTLLRPD GSSAELWTGI KKGPPRKLKF PEPQEVVEEL KKYLS.
