BUD31 Human

BUD31 plays a critical role in alternative splicing, a post-transcriptional process that generates multiple mRNA variants from a single gene, thereby contributing to proteomic diversity and cellular complexity . This process is essential for the regulation of gene expression and the production of protein isoforms that can have distinct functions.

Recent studies have highlighted the significance of BUD31 in various cancers. For instance, BUD31 has been found to be upregulated in multiple tumors, including clear cell renal cell carcinoma (ccRCC) . High BUD31 expression is correlated with worse survival outcomes and increased genomic instability . It has also been identified as an independent predictor of poor prognosis in ccRCC . BUD31 promotes cell cycle progression via alternative splicing, suggesting its potential as a prognostic biomarker and therapeutic target in cancer .

Recombinant human BUD31 protein is typically expressed in Escherichia coli and purified using conventional chromatography techniques . The recombinant protein often includes a His-tag at the N-terminus to facilitate purification . The theoretical molecular weight of the recombinant BUD31 protein is approximately 19.4 kDa . It is used in various research applications, including studies on its role in transcription regulation and cancer progression.

Recombinant human BUD31 protein should be stored at 4°C for short-term use and at -20°C for long-term storage to avoid freeze-thaw cycles . It is typically supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 40% glycerol, and 2 mM DTT .