BUB3 Human

BUB3 contains four WD repeat domains, which are motifs involved in protein-protein interactions. These domains are essential for the kinetochore localization of other checkpoint proteins such as BUB1 and BUBR1 . The kinetochore is a critical structure on chromosomes that ensures proper attachment to the spindle microtubules during cell division.

The primary function of BUB3 is to regulate the establishment of correct kinetochore-microtubule attachments. This regulation is vital for maintaining genomic stability and preventing aneuploidy, a condition where cells have an abnormal number of chromosomes .

Recombinant human BUB3 protein is typically produced in E. coli and is often fused to a His-tag at the N-terminus to facilitate purification. The recombinant protein corresponds to the amino acids 1-328 of the human BUB3 sequence . The molecular mass of this recombinant protein is approximately 39.5 kDa .

The recombinant BUB3 protein is used in various research applications, including studies on cell cycle regulation, cancer research, and the development of therapeutic interventions targeting the mitotic checkpoint pathway .

Recombinant BUB3 protein is used in SDS-PAGE and other biochemical assays to study its interactions and functions. It is typically formulated in a buffer containing Tris-HCl, urea, and glycerol, and should be stored at -20°C for long-term stability .