BTN3A1 Human
Sf9, Baculovirus cells.
Butyrophilin Subfamily 3 Member A1, BTF5, DJ45P21.3 (Butyrophilin, Subfamily 3, Member A1), Butyrophilin, Subfamily 3, Member A1, CD277 Antigen, BTN3.1, BT3.1, CD277, Butyrophilin subfamily 3 member A1.
Greater than 90.0% as determined by SDS-PAGE.
Description
BTN3A1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 464 amino acids (30-254a.a.) and having a molecular mass of 51.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
BTN3A1 is expressed with a 239 amino acid hIgG-His Tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Butyrophilin subfamily 3 member A1, also known as BTN3A1, is a protein belonging to the immunoglobulin superfamily. Structurally, it comprises an extracellular N-terminal IgV domain, a membrane-proximal IgC domain, a transmembrane domain, and a cytoplasmic tail. Functionally, BTN3A1 plays a crucial role in T-cell activation and the adaptive immune response. It regulates the proliferation of activated T-cells and influences the release of cytokines, including IFNG, by these cells. Importantly, BTN3A1 mediates the response of T-cells to infected or transformed cells characterized by elevated levels of phosphorylated metabolites like isopentenyl pyrophosphate.
Recombinant human BTN3A1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 464 amino acids (30-254a.a.). It has a molecular mass of 51.1 kDa. However, on SDS-PAGE, its apparent molecular size ranges from approximately 50 to 70 kDa. The protein is expressed with a 239 amino acid hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
The BTN3A1 protein solution is provided at a concentration of 0.5 mg/ml in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
The purity of the BTN3A1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Butyrophilin Subfamily 3 Member A1, BTF5, DJ45P21.3 (Butyrophilin, Subfamily 3, Member A1), Butyrophilin, Subfamily 3, Member A1, CD277 Antigen, BTN3.1, BT3.1, CD277, Butyrophilin subfamily 3 member A1.
Sf9, Baculovirus cells.
QFSVLGPSGP ILAMVGEDAD LPCHLFPTMS AETMELKWVS SSLRQVVNVY ADGKEVEDRQ SAPYRGRTSI LRDGITAGKA ALRIHNVTAS DSGKYLCYFQ DGDFYEKALV ELKVAALGSD LHVDVKGYKD GGIHLECRST GWYPQPQIQW SNNKGENIPT VEAPVVADGV GLYAVAASVI MRGSSGEGVS CTIRSSLLGL EKTASISIAD PFFRSAQRWI AALAGLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.
Product Science Overview
BTN3A1 is a type I membrane protein characterized by two extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain . The protein plays a crucial role in the immune system, particularly in T-cell activation and the adaptive immune response . It regulates the proliferation of activated T-cells and the release of cytokines, including interferon-gamma (IFNG), by these cells . BTN3A1 mediates the response of T-cells towards infected and transformed cells that exhibit high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate .
Human recombinant BTN3A1 is used in research to study its function in the immune system and its potential as a therapeutic target. The recombinant protein is produced using recombinant DNA technology, which involves inserting the BTN3A1 gene into an expression system to produce the protein in vitro.
