BTLA Mouse

B and T Lymphocyte Associated Mouse Recombinant
Cat. No.
BT28901
Source

HEK293 Cells

Synonyms

B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, CD272, B And T Lymphocyte Associated, B- And T-Lymphocyte-Associated Protein, B- And T, Lymphocyte Attenuator, CD272 Antigen, BTLA1

Appearance

Sterile filtered colorless solution.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BTLA Mouse Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 390 amino acids (30-176 a.a) and having a molecular mass of 44.1 kDa.
BTLA is fused to a 239 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
BTLA, also known as B & T Lymphocyte Associated, is an inhibitory molecule that belongs to the Ig superfamily. It is a type 1 transmembrane glycoprotein and a member of the CD28 family of T cell costimulatory molecules. BTLA acts as the third inhibitory receptor on T lymphocytes, sharing similarities with CTLA-4 and PD-1. Furthermore, BTLA serves as a ligand for TNFRSF14 (a TNF receptor superfamily member) and HVEM. The interaction between BTLA and HVEM forms complexes that negatively regulate T-cell immune responses.
Description
Recombinant BTLA Mouse, produced in HEK293 cells, is a single, glycosylated polypeptide chain. This protein consists of 390 amino acids (specifically, amino acids 30 to 176) and has a molecular weight of 44.1 kDa. A 239 amino acid hIgG-His-Tag is fused to the C-terminus of BTLA. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The BTLA solution is provided at a concentration of 0.25 mg/mL in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms

B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, CD272, B And T Lymphocyte Associated, B- And T-Lymphocyte-Associated Protein, B- And T, Lymphocyte Attenuator, CD272 Antigen, BTLA1

Source

HEK293 Cells

Amino Acid Sequence

DGSMEKATKR NDEECEVQLN IKRNSKHSAW TGELFKIECP VKYCVHRPNV TWCKHNGTIW VPLEVGPQLY TSWEENRSVP VFVLHFKPIH LSDNGSYSCS TNFNSQVINS HSVTIHVRER TQNSSEHPLI ISDIPDATNA SGPSTMEKRP GLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH

Product Science Overview

Structure and Function

BTLA is composed of four extracellular cysteine-rich domains . It is an inhibitory molecule that negatively regulates T cell activation and proliferation. Unlike CTLA-4 and PD-1, which bind to B7 family members, BTLA interacts with HVEM . This interaction was the first demonstration of crosstalk between the immunoglobulin and TNFR superfamilies . The BTLA/HVEM interaction leads to the phosphorylation of BTLA tyrosine residues, inhibiting T-cell proliferation and modulating immune responses .

Expression and Regulation

BTLA is expressed on various immune cells, including T cells, B cells, macrophages, dendritic cells, and natural killer cells . Its expression levels are low on resting T cells but increase upon T cell activation . In contrast, BTLA is highly expressed on resting B cells .

Recombinant Mouse BTLA

Recombinant Mouse BTLA is produced in HEK293 cells and is available in various forms, including BTLA-Fc chimeras and His-tagged proteins . These recombinant proteins are used in research to study the BTLA/HVEM interaction and its role in immune regulation. The recombinant proteins are typically purified using chromatographic techniques and are characterized by their high purity and specific activity .

Applications in Research

The study of BTLA and its interactions with HVEM has significant implications for understanding immune regulation and developing therapeutic strategies for autoimmune diseases, infections, and cancer . By modulating the BTLA/HVEM pathway, researchers aim to enhance or suppress immune responses as needed for various clinical applications .

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