BST1 Human
Sf9, Baculovirus cells.
Bone Marrow Stromal Cell Antigen 1, ADP-Ribosyl Cyclase 2, Bone Marrow Stromal Antigen 1, Cyclic ADP-Ribose Hydrolase 2, NAD(+) Nucleosidase, CADPr Hydrolase 2, ADP-Ribosyl Cyclase/Cyclic ADP-Ribose Hydrolase 2, CD157 Antigen, EC 3.2.2.6, CD157, BST-1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2, Bone marrow stromal antigen 1, Cyclic ADP-ribose hydrolase 2, cADPr hydrolase 2.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
BST1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 267 amino acids (33-293a.a.) and having a molecular mass of 30.5kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
BST1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
BST1 (Bone Marrow Stromal Cell Antigen 1) is a cell surface protein belonging to the CD38 family and is anchored to the cell membrane by a GPI (glycosylphosphatidylinositol) anchor. Initially identified on bone marrow stromal cells, BST1 acts as an ectoenzyme, similar to ADP-ribosyl cyclase CD38. Both BST1 and CD38 possess DP-ribosyl cyclase and cyclic ADP ribose hydrolase activities. Increased expression of BST1 in rheumatoid arthritis (RA) suggests its involvement in the disease, particularly through its elevated presence in RA-derived bone marrow stromal cell lines. BST1 is also found on myeloid lineage cells and is thought to function as a receptor with signal transduction capabilities.
Produced in Sf9 insect cells using a baculovirus expression system, BST1 is a single-chain polypeptide containing 267 amino acids (residues 33-293). A 6-amino acid Histidine tag is present at the C-terminus to facilitate purification. The protein has a molecular weight of 30.5 kDa, but due to glycosylation, it appears as a band of approximately 40-57 kDa on SDS-PAGE. Purification is achieved using proprietary chromatographic techniques.
The product is a clear, colorless, and sterile-filtered solution.
BST1 is supplied as a 0.5 mg/mL solution in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
The purity of BST1 is greater than 95%, as determined by SDS-PAGE analysis.
Bone Marrow Stromal Cell Antigen 1, ADP-Ribosyl Cyclase 2, Bone Marrow Stromal Antigen 1, Cyclic ADP-Ribose Hydrolase 2, NAD(+) Nucleosidase, CADPr Hydrolase 2, ADP-Ribosyl Cyclase/Cyclic ADP-Ribose Hydrolase 2, CD157 Antigen, EC 3.2.2.6, CD157, BST-1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2, Bone marrow stromal antigen 1, Cyclic ADP-ribose hydrolase 2, cADPr hydrolase 2.
Sf9, Baculovirus cells.
RWRGEGTSAH LRDIFLGRCA EYRALLSPEQ RNKNCTAIWE AFKVALDKDP CSVLPSDYDL FINLSRHSIP RDKSLFWENS HLLVNSFADN TRRFMPLSDV LYGRVADFLS WCRQKNDSGL DYQSCPTSED CENNPVDSFW KRASIQYSKD SSGVIHVMLN GSEPTGAYPI KGFFADYEIP NLQKEKITRI EIWVMHEIGG PNVESCGEGS MKVLEKRLKD MGFQYSCIND YRPVKLLQCV DHSTHPDCAL KSAAAATQRK AHHHHHH.
Product Science Overview
Bone Marrow Stromal Cell Antigen 1 (BST1), also known as CD157, is a glycosylphosphatidylinositol (GPI)-anchored molecule that plays a significant role in the regulation of pre-B cell growth. It is a member of the ADP-ribosyl cyclase family of enzymes, which are involved in the metabolism of nicotinamide adenine dinucleotide (NAD+). BST1 is encoded by the BST1 gene located on chromosome 4 in humans .
BST1 is structurally similar to CD38, another member of the ADP-ribosyl cyclase family, with a 33% similarity in their amino acid sequences . The enzyme catalyzes the formation of cyclic ADP-ribose (cADPR) from NAD+, although it is a weaker catalyst compared to CD38 . cADPR is crucial for the regulation of calcium ion (Ca2+) signaling within cells .
BST1 is predominantly expressed in bone marrow stromal cells, particularly in those derived from patients with rheumatoid arthritis . The overexpression of BST1 in these cells is believed to contribute to the polyclonal B cell abnormalities observed in rheumatoid arthritis . Additionally, BST1 is expressed in various other tissues, including monocytes, granulocytes, and certain epithelial cells .
BST1 has several biological functions, including:
- Regulation of Pre-B Cell Growth: BST1 facilitates the growth and differentiation of pre-B cells, which are essential for the development of the immune system .
- Immune Response: BST1 is involved in the regulation of the humoral immune response, which is mediated by antibodies produced by B cells .
- Cell Adhesion and Migration: BST1 plays a role in cell-matrix adhesion and the regulation of cellular extravasation, which is the movement of cells out of blood vessels into tissues .
The expression of BST1 is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis, suggesting a potential role in the pathogenesis of this autoimmune disease . Additionally, BST1 has been implicated in various other conditions, including certain cancers and inflammatory diseases .
