BRMS1 Human

Breast Cancer Metastasis Suppressor 1 Human Recombinant
Cat. No.
BT28672
Source
Escherichia Coli.
Synonyms
Breast Cancer Metastasis Suppressor 1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage

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Description

BRMS1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 261 amino acids (1-246) and having a molecular mass of 29.9 kDa. BRMS1 is fused to a 15 amino acid T7-tag at N-terminus.

Product Specs

Introduction
BRMS1 protein belongs to the mSin3a family of histone deacetylase complexes (HDAC) and is primarily found in the nucleus. It possesses two coiled-coil motifs and several imperfect leucine zipper motifs. BRMS1 has been shown to suppress the metastatic potential of melanoma and human breast cancer cell lines without affecting their tumorigenicity. Alternative splicing results in two transcript variants that encode distinct isoforms.
Description
Recombinant BRMS1 protein, produced in E.coli, is a single, non-glycosylated polypeptide chain consisting of 261 amino acids (residues 1-246). It has a molecular weight of 29.9 kDa. The protein includes a 15 amino acid T7-tag fused to its N-terminus.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The BRMS1 solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 80% by SDS-PAGE analysis.
Synonyms
Breast Cancer Metastasis Suppressor 1.
Source
Escherichia Coli.
Amino Acid Sequence
MASMTGGQQM GRGSHMPVQP PSKDTEEMEA EGDSAAEMNG EEEESEEERS GSQTESEEES SEMDDEDYER RRSECVSEML DLEKQFSELK EKLFRERLSQ LRLRLEEVGA ERAPEYTEPL GGLQRSLKIR IQVAGIYKGF CLDVIRNKYE CELQGAKQHL ESEKLLLYDT LQGELQERIQ RLEEDRQSLD LSSEWWDDKL HARGSSRSWD SLPPSKRKKA PLVSGPYIVY MLQEIDILED WTAIKKARAA VSPQKRKSDG P

Product Science Overview

Introduction

Breast Cancer Metastasis Suppressor 1 (BRMS1) is a protein that plays a crucial role in inhibiting the metastasis of breast cancer cells. Metastasis is the process by which cancer cells spread from the primary tumor to distant organs, leading to the formation of secondary tumors. This process is responsible for the majority of cancer-related deaths. BRMS1 has been identified as a key player in suppressing this process, making it a significant focus of cancer research.

Discovery and Gene Location

BRMS1 was discovered in the 1990s through studies that observed a correlation between deletions in chromosome 11 and increased cancer aggressiveness in breast cancer patients . The BRMS1 gene is located on chromosome 11q13.1-q13.2 . It was found that the introduction of a normal human chromosome 11 into metastatic breast cancer cells significantly reduced their metastatic potential without affecting their ability to form primary tumors .

Structure and Isoforms

The BRMS1 gene encodes a protein consisting of 246 amino acids . There are also isoforms of BRMS1, including proteins with 290 and 321 amino acids, as well as a BRMS1-homologue protein . These isoforms may have varying roles in the suppression of metastasis.

Mechanisms of Action

BRMS1 functions as part of the mSin3-HDAC (histone deacetylase) transcription co-repressor complex . This complex is involved in chromatin remodeling, which regulates the expression of various genes. BRMS1 has been implicated in several signaling pathways, including focal adhesion kinase (FAK), epidermal growth factor receptor (EGFR), and NF-κB signaling pathways . These pathways are crucial for cell migration, invasion, and survival, all of which are key steps in the metastatic process.

Role in Cancer Suppression

BRMS1 has demonstrated a variety of effects on cell functions, such as reducing cell migration, invasiveness, angiogenesis, and enhancing cell adhesion . It also modulates the immune recognition of cancer cells. These effects collectively contribute to its robust anti-metastatic influence. BRMS1 has been shown to suppress metastasis not only in breast cancer but also in other cancers, including non-small cell lung cancer, ovarian cancer, melanoma, and rectal cancer .

Clinical Relevance

Recent clinical studies have confirmed that BRMS1 can be used as a prognostic marker for cancer progression . Its expression levels are positively correlated with patient outcomes, making it a potential target for therapeutic interventions. Approaches to develop anti-cancer treatments that leverage BRMS1’s mechanisms are currently being explored .

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