BRK1 Human
BRK1 Human Recombinant
Cat. No.
BT28585
Source
Escherichia Coli.
Synonyms
BRICK1 SCAR/WAVE Actin-Nucleating Complex Subunit, Haematopoietic Stem/Progenitor Cell Protein 300, Homolog (Arabidopsis Thaliana), Chromosome 3 Open Reading Frame 10, Probable Protein BRICK1, BRICK1, HSPC300, MDS027, C3orf10.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
BRK1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 98 amino acids (1-75) and having a molecular mass of 11.0 kDa. BRK1 is fused to a 23 amino acid His-tag at N-terminus.
Product Specs
Introduction
As a member of the BRK1 family, BRK1 plays a role in the organization of actin and microtubules. It is part of a WAVE complex responsible for activating the Arp2/3 complex.
Description
Recombinant human BRK1, produced in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 11.0 kDa. It consists of 98 amino acids, including amino acids 1 to 75 of the BRK1 sequence, and a 23 amino acid His-tag fused at the N-terminus.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The BRK1 solution is provided at a concentration of 0.5 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 150 mM NaCl, and 20% glycerol.
Stability
For optimal storage, keep the entire vial at 4°C if it will be used within 2-4 weeks. For longer-term storage, freeze the solution at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for extended storage. It's important to minimize repeated freezing and thawing of the solution.
Purity
The purity of the protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
BRICK1 SCAR/WAVE Actin-Nucleating Complex Subunit, Haematopoietic Stem/Progenitor Cell Protein 300, Homolog (Arabidopsis Thaliana), Chromosome 3 Open Reading Frame 10, Probable Protein BRICK1, BRICK1, HSPC300, MDS027, C3orf10.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGQEDP VQREIHQDWA NREYIEIITS SIKKIADFLN SFDMSCRSRL ATLNEKLTAL ERRIEYIEAR VTKGETLT
Product Science Overview
Gene and Protein Information
- Gene Name: BRK1 (C3orf10, HSPC300, MDS027)
- Protein Class: BRK1 is classified under the BRK1 family and is involved in actin nucleation .
- Protein Structure: The recombinant human BRK1 protein is produced in Escherichia coli as a single, non-glycosylated polypeptide chain containing 98 amino acids (1-75) with a molecular mass of 11.0 kDa. It is fused to a 23 amino acid His-tag at the N-terminus .
Function and Localization
BRK1 is involved in the regulation of the actin cytoskeleton, which is critical for maintaining cell shape and enabling cellular movements. It is localized primarily to the nuclear speckles and cell junctions . The protein interacts with multiple other proteins, forming complexes that are essential for its function.
Expression and Stability
- Expression: BRK1 is expressed in various tissues, including the brain, lung, liver, and kidney .
- Stability: The recombinant BRK1 protein is stable when stored at 4°C for short-term use (2-4 weeks) and at -20°C for long-term storage. It is recommended to add a carrier protein for extended storage to prevent degradation .
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