BRD3 Human
Greater than 90.0% as determined by SDS-PAGE.
Description
BRD3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Bromodomain-containing proteins (BRD proteins) are a family of proteins that play crucial roles in the regulation of gene expression through their interaction with acetylated lysine residues on histone and non-histone proteins . Among these, Bromodomain Containing 3 (BRD3) is a significant member, known for its involvement in various cellular processes.
BRD3, also known as RING3L, is characterized by the presence of bromodomains, which are evolutionarily conserved protein modules consisting of approximately 110 amino acids . These bromodomains act as specific readers for acetylated lysine residues, facilitating the regulation of transcription, chromatin remodeling, DNA damage repair, and cell proliferation .
BRD3 contains two bromodomains and is part of the bromodomain and extra-terminal (BET) family, which also includes BRD2, BRD4, and BRDT . The BET family proteins are known for their role in recognizing acetylated lysines on histones, thereby influencing gene expression and chromatin structure.
BRD3 functions as a scaffolding protein, assembling multi-protein complexes to regulate diverse biological processes . It is involved in transcriptional regulation by binding to acetylated histones and recruiting other transcriptional machinery components. This interaction is crucial for the activation or repression of specific genes, impacting various cellular functions such as cell cycle control, cell metabolism, and nuclear transport .
Recombinant BRD3 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the gene encoding BRD3 into an expression system, such as E. coli, to produce the protein in large quantities . Recombinant BRD3 is often tagged with a histidine (His) tag to facilitate purification and detection .
BRD3 and other bromodomain-containing proteins have been the focus of extensive research due to their involvement in various diseases, including cancers, inflammation, cardiovascular diseases, and viral infections . Inhibitors targeting BRD3 and other BET proteins have shown promise in preclinical and clinical studies as potential therapeutic agents . These inhibitors work by disrupting the interaction between bromodomains and acetylated lysines, thereby modulating gene expression and offering a novel approach to disease treatment.
