BRD2 Human

Bromodomain Containing 2 Human Recombinant
Cat. No.
BT27298
Source
Escherichia Coli.
Synonyms
Bromodomain Containing 2, RING3, Really Interesting New Gene 3 Protein, Bromodomain-Containing 2, O27.1.1, FSRG1, Female Sterile Homeotic-Related Gene 1, Bromodomain-Containing Protein 2, KIAA9001, D6S113E, RNF3, FSH, NAT, Bromodomain-containing protein 2 .
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BRD2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 478 amino acids (1-455 a.a) and having a molecular mass of 52.8kDa.
BRD2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Bromodomain Containing 2, also known as BRD2, is a transcriptional regulator belonging to the BET (bromodomains and extra terminal domain) family of proteins. BRD2 interacts with transcription complexes and acetylated chromatin during mitosis. It specifically binds to the acetylated lysine-12 residue of histone H4 through its two bromodomains. The gene encoding BRD2 is located within the major histocompatibility complex (MHC) class II region on chromosome 6p21.3; however, sequence comparisons suggest that the protein is not involved in the immune response. BRD2 has been linked to juvenile myoclonic epilepsy, a prevalent form of epilepsy that typically emerges during adolescence.
Description
Recombinant human BRD2 protein was expressed in E. coli as a single, non-glycosylated polypeptide chain consisting of 478 amino acids (residues 1-455) with a molecular weight of 52.8 kDa. The protein includes a 23 amino acid His-tag at the N-terminus. Purification was achieved using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The BRD2 protein solution (1 mg/ml) is supplied in phosphate-buffered saline (pH 7.4) containing 10% glycerol and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), store the vial at 4°C. For long-term storage, freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90.0% as assessed by SDS-PAGE.
Synonyms
Bromodomain Containing 2, RING3, Really Interesting New Gene 3 Protein, Bromodomain-Containing 2, O27.1.1, FSRG1, Female Sterile Homeotic-Related Gene 1, Bromodomain-Containing Protein 2, KIAA9001, D6S113E, RNF3, FSH, NAT, Bromodomain-containing protein 2 .
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLQNVTP HNKLPGEGNA GLLGLGPEAA APGKRIRKPS LLYEGFESPT MASVPALQLT PANPPPPEVS NPKKPGRVTN QLQYLHKVVM KALWKHQFAW PFRQPVDAVK LGLPDYHKII KQPMDMGTIK RRLENNYYWA ASECMQDFNT MFTNCYIYNK PTDDIVLMAQ TLEKIFLQKV ASMPQEEQEL VVTIPKNSHK KGAKLAALQG SVTSAHQVPA VSSVSHTALY TPPPEIPTTV LNIPHPSVIS SPLLKSLHSA GPPLLAVTAA PPAQPLAKKK GVKRKADTTT PTPTAILAPG SPASPPGSLE PKAARLPPMR RESGRPIKPP RKDLPDSQQQ HQSSKKGKLS EQLKHCNGIL KELLSKKHAA YAWPFYKPVD ASALGLHDYH DIIKHPMDLS TVKRKMENRD YRDAQEFAAD VRLMFSNCYK YNPPDHDVVA MARKLQDVFE FRYAKMPD.

Product Science Overview

Introduction

Bromodomain-containing proteins are a group of proteins that play a crucial role in regulating gene expression by recognizing and binding to acetylated lysine residues on histone and non-histone proteins. Among these, Bromodomain Containing 2 (BRD2) is a significant member of the BET (Bromodomain and Extra-Terminal domain) family, which also includes BRD3, BRD4, and BRDT .

Structure and Function

BRD2 contains two tandem bromodomains (BD1 and BD2) at its N-terminus and a conserved ET (Extra-Terminal) domain at its C-terminus . These bromodomains are structurally conserved epigenetic reader domains that bind to acetylated lysine residues, facilitating the regulation of gene expression . The two bromodomains of BRD2 have distinct roles:

  • BD1: Known for its role in binding acetylated histone H4, contributing to chromatin remodeling and transcriptional regulation.
  • BD2: Although similar to BD1, BD2 has unique structural features, including a distinctive ZA loop that forms part of the acetyl-lysine binding site .
Biological Significance

BRD2 is involved in various biological processes, including cell cycle regulation, transcriptional activation, and chromatin remodeling . It acts as a scaffold protein, assembling multi-protein complexes that regulate diverse biological pathways . The interaction of BRD2 with acetylated histones is crucial for the regulation of gene expression, particularly during cell division and differentiation .

Research and Applications

Research on BRD2 has provided insights into its role in various diseases, including cancer and inflammatory disorders. The development of small molecule inhibitors targeting BRD2 and other BET proteins has shown promise in preclinical studies for treating these conditions . Human recombinant BRD2 is used in research to study its structure, function, and interactions with other proteins, aiding in the development of therapeutic strategies.

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